Animals were immunized with ICAM-1 transfected FT16.I1 cells. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody blocks the binding of lymphocyte function antigen 1 (LFA-1, CD11a, CD18) to ICAM-1. The antiboy is useful for studies of inflammation, lymphocyte differentiation and lymphocyte activation. It can be used for purification of ICAM-1 and for staining of cell surface ICAM-1 by flow cytometry.
Application Notes
Purification of ICAM-1 and staining of cell surface ICAM-1 by FC.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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