Animals were immunized with NS-1 mouse myeloma cells. Spleen cells were fused with Y3 rat myeloma cells. The antibody reacts with a 95000 to 100000 dalton antigen (ICAM-1) on activated and proliferating murine lymphocytes. The antibody partially inhibits Con A stimulation of spleen cells but does not inhibit LPS stimulation. The antibody strongly inhibits mixed lymphocyte responses (MLR). The ICAM-1 antigen is absent on most unstimulated thymocytes and spleen cells.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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