Rabies virus Nucleoprotein ELISA Kit

Sample

Vaccine samples

Species Reactivity

Rabies Virus

Detection Method

sELISA

Intended Use

This kit is developed for quantitative detection of Rabies virus Nucleoprotein in samples. It is intended for research use only (RUO).

Contents of Kit

1. Pre-coated Anti-Nucleoprotein (RABV) Antibody Microplate, 1 plate. Unopened: 2-8°C. Opened: 2-8°C.
2. Nucleoprotein (RABV) Standard, 15 μg. Powder. Unopened: 2-8°C. Opened: -70°C.
3. Biotin-Anti-Nucleoprotein (RABV) Antibody, 15 μg. Powder. Unopened: 2-8°C. Opened: -70°C.
4. Streptavidin-HRP, 15 μg. Powder. Unopened: 2-8°C, avoid light. Opened: -70°C, avoid light.
5. 10×Washing Buffer, 50 mL. Liquid. Unopened: 2-8°C. Opened: 2-8°C.
6. 2×Dilution Buffer, 50 mL. Liquid. Unopened: 2-8°C. Opened: 2-8°C.
7. Substrate Solution, 12 mL. Liquid. Unopened: 2-8°C, avoid light. Opened: 2-8°C, avoid light.
8. Stop Solution, 7 mL. Liquid. Unopened: 2-8°C. Opened: 2-8°C.

Storage

1. Unopened kit should be stored at 2°C-8°C upon receiving.
2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.
3. The opened kit should be stored as Reagents And Materials Provided. The shelf life is 30 days from the date of opening.

Precision

1. Intra-assay Precision
Three samples of known concentration were tested ten times on one plate to assess intra-assay precision.
2. Inter-assay Precision
Three samples of known concentration were tested in three separate assays to assess inter-assay precision.

Note: The example data is for reference only.

General Description

Rabies virus (RABV), scientific name Rabies lyssavirus, is a deadly neurotropic virus that causes rabies in humans and animals. Rabies virus has an extremely wide host range and its transmission most often occurs through the saliva of animals. Without intervention prior to disease progression, rabies has the highest case fatality of any infectious disease. RABV contains a single-stranded negative-sense R genome that encodes five structural proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and R-dependent R polymerase (L). Among these viral proteins, the RABV glycoprotein (RABV-G) is a pivotal player mediating virus entry and the major target of neutralizing antibodies, thus a key factor for vaccine and drug design.A rapid and effective assay kit detecting the levels of RABV-N is urgently needed to accelerate the development of RABV vaccines.

Standard Curve

General Notes

Rabies virus (RABV), scientific name Rabies lyssavirus, is a deadly neurotropic virus that causes rabies in humans and animals. Rabies virus has an extremely wide host range and its transmission most often occurs through the saliva of animals. Without intervention prior to disease progression, rabies has the highest case fatality of any infectious disease. RABV contains a single-stranded negative-sense R genome that encodes five structural proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and R-dependent R polymerase (L). Among these viral proteins, the RABV glycoprotein (RABV-G) is a pivotal player mediating virus entry and the major target of neutralizing antibodies, thus a key factor for vaccine and drug design.A rapid and effective assay kit detecting the levels of RABV-N is urgently needed to accelerate the development of RABV vaccines.