The Human anti- Rabies Virus Glycoprotein (RVG) IgG ELISA Kit is an immunoassay suitable for detecting and quantifying IgG antibody activity specific for RVG, in serum or plasma. Other biological fluids, including tissue culture medium, may be validated for use. For research use only (RUO), not for diagnosis, cure or prevention of the disease.
The unopened kit is stable at 2-8°C until the expiration date printed on the box label. The redissolved standards must be stored at ≤ -20°C and be stable for 3 months.
Precision
U/mL
Intra CV
Inter CV
10
7%
8%
5
4%
10%
2.5
3%
8%
1
8%
9%
Sensitivity
The RVG antigen coating level and HRP conjugate concentration are optimized to differentiate anti-RVG IgG from background (non-antibody) signal with human serum or plasma samples diluted 1:100. Notes The sensitivity of the assay may be increased to perhaps convert a borderline sample to a positive by using a lower dilution of the sample, e.g., 1/50. The values of negatives may increase, so an alternative threshold should be established using known negatives to develop a Positive Index, or by using known Internal Controls as discriminator for a Threshold Control (instead of the kit 1 U/ml Calibrator Control)
General Description
Rabies is a fatal zoonotic disease of serious public health and economic significance worldwide. The rabies virus glycoprotein (RVG) has been the major target for subunit vaccine development, since it harbors domains responsible for induction of virus-neutralizing antibodies, infectivity, and neurovirulence.
Standard Curve
U/mL
OD1
OD2
10
3.2071
2.9014
5
1.8983
1.6531
2.5
0.8349
0.8083
1
0.4261
0.3861
0
0.0141
0.016
Positive Control
0.6609
0.6484
General Notes
Infectious Disease ELISA Kits
Citations
Publication ()
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Background
Rabies is a zoonotic disease caused by the rabies virus and is prevalent in many countries and regions around the world. Once it occurs, the fatality rate is almost 100%, making it the acute infectious disease with the highest fatality rate in humans. Rabies virus belongs to the family Rhabdoviridae and the genus Lyssavirus, is an enveloped single negative-strand RNA virus, with a genome consisting of 11,928-11,932 nucleotides, including 5 large The open reading frame encodes five structural proteins. The sequence of the genome from the 3' end to the 5' end is N, M1, M2, G and L genes, which respectively encode viral nucleoprotein (NP), capsid matrix protein (M1P), Membrane matrix protein (M2P), glycoprotein (GP) and retroprotein (LP), among which GP is the most widely and deeply studied protein among the structural proteins of rabies virus. Rabies virus GP (RVG) is a transmembrane glycoprotein that exists on the surface of the virus envelope in the form of a homotrimer, forming protrusions on the virus surface. It is the only antigen among the five structural proteins of rabies virus that can stimulate the body to produce neutralizing antibodies, and is related to various biological characteristics of rabies virus.
Figure 1. Structural Analysis of Rabies Virus Glycoprotein.( Source: Yang F, et al., 2020)
Since we need to find ways to prevent and treat rabies virus, research on RVG is necessary. RVG detection is required in the process of developing new rabies vaccines, evaluating new vaccines, detecting antigens, and evaluating rabies neutralizing antibody levels. ELISA kit testing is a convenient and fast testing method. The Human Anti-Rabies Virus Glycoprotein (RVG) IgG ELISA Kit is an immunoassay suitable for the detection and quantification of RVG-specific IgG antibody activity in serum, plasma, tissue culture media, and other biological fluids. It can help detect the amount of rabies virus in samples.
1. Yang F, et al., Structural Analysis of Rabies Virus Glycoprotein Reveals pH-Dependent Conformational Changes and Interactions with a Neutralizing Antibody. Cell Host Microbe. 2020, 27(3):441-453.e7.
Q: Do you have any traceability standards for the kit as per the WHO standards?
A: The calibrator in Cat# DEIAHRVPY28 was calibrated using the 2nd WHO International Human Anti-rabies Std (NIBSC, RAI).
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References
The cell biology of rabies virus: using stealth to reach the brain
Rabies virus, the prototypical neurotropic virus, causes one of the most lethal zoonotic diseases. According to official estimates, over 55,000 people die of the disease annually, but this is probably a severe underestimation. A combination of virulence factors enables the virus to enter neurons at peripheral sites and travel through the spinal cord to the brain of the infected host, where it often induces aggression that facilitates the transfer of the virus to a new host. This Review summarizes the current knowledge of the replication cycle of rabies virus and virus- host cell interactions, both of which are fundamental elements in our quest to understand the life cycle of rabies virus and the pathogenesis of rabies.
Rabies Infection: An Overview of Lyssavirus-Host Protein Interactions
Viruses are obligatory intracellular parasites that use cell proteins to take the control of the cell functions in order to accomplish their life cycle. Studying the viral-host interactions would increase our knowledge of the viral biology and mechanisms of pathogenesis. Studies on pathogenesis mechanisms of lyssaviruses, which are the causative agents of rabies, have revealed some important host protein partners for viral proteins, especially for most studied species, i.e. Rabies virus. In this review article, the key physical lyssavirus-host protein interactions, their contributions to rabies infection, and their exploitation are discussed to improve the knowledge about rabies pathogenesis.
COA
Publication 
Figure 1. Structural Analysis of Rabies Virus Glycoprotein.( Source: Yang F, et al., 2020)
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