G. Pig Anti-Rabies Virus IgG ELISA Kit

Sample

serum or plasma

Species Reactivity

Guinea pig

Detection Method

iELISA

Intended Use

The Guinea Pig Anti-Rabies virus IgG ELISA Kit detects and quantifies rabies virus-specific IgG in serum or plasma of vaccinated, immunized and/or infected hosts. This immunoassay is suitable for:
1. Determining immune status relative to non-immune controls;
2. Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing;
3. Qualifying and standardizing vaccine batches & protocols. This kit is for research use only (RUO) and not for diagnosis cure or prevention of the disease.

Storage

The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label.

Performance Characteristics

Calibrator Values
The Calibrators are dilutions of anti-rabies antibody. Values are assigned as arbitrary anti-rabies virus activity units (see Limits of the Assay).

Sensitivity

The rabies-coated plate, anti-G. Pig IgG HRP concentration, and Low NSB Sample Diluent are optimized to differentiate anti-rabies IgG from background (non-antibody) signal with G. Pig serum samples diluted 1:100.

General Description

Rabies is a fatal zoonotic disease of serious public health. The rabies virus is a member of the Lyssavirus genus, which have helical symmetry. The lipoprotein envelope carries knob-like spikes composed of Glycoprotein G (VRG). Beneath the envelope is the membrane or matrix (M) protein layer. The core of the virion consists of helically arranged ribonucleoprotein (RV-NP). Old vaccines are made from whole inactivated virus. New recombinant subunit vaccines are based upon purified proteins (RV-NP or VRG) that invoke strong antibodies against the rabies virus. The V-RG vaccine (Raboral/Merial) is harmless to humans and safe for various species of domestic or wild animals.