Human Anti-Rabies NP IgG ELISA Kit

Sample

Serum/Plasma/Biological Samples

Species Reactivity

Human

Detection Method

iELISA

Intended Use

The Human anti-Rabies Virus NP (Nucleoprotein) IgG ELISA Kit is an immunoassay suitable for quantifying IgG antibody activity specific for Rabies NP in serum or plasma. This immunoassay is suitable for:
1. Determining immune status relative to non-immune controls
2. Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization, and timing
3. Qualifying and standardizing vaccine batches & protocols
Reagents contain no virus or viral antigens. These kits are designed for studying efficacy of existing vaccines and preparations of more effective rabies vaccine formulations.The assay is for research use only (RUO).

Storage

The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label.

General Description

Rabies is a fatal zoonotic disease of serious public health concern. The rabies virus is a member of the Lyssavirus genus which have helical symmetry. The lipoprotein envelope carries knob-like spikes composed of Glycoprotein G (VRG). Beneath the envelope is the membrane or matrix (M) protein layer. The core of the virion consists of helically arranged ribonucleoprotein (RV-NP). Old vaccines are made from whole inactivated virus. New recombinant subunit vaccines are based upon purified proteins (RV-NP or VRG) that invoke strong antibodies against the rabies virus. The V-RG vaccine (Raboral/Merial) is harmless to humans and safe for various species of domestic or wild animals.

Standard Curve

NOTE: This data is for demonstration purpose only. A complete standard curve must be run in every assay to determine sample values. Blank should not be used in calculation of the curve, only to normalize standard and sample values. Each laboratory should determine their own normal reference values.

A typical assay standard curve (do not use this for calculating sample values)