Monkey Anti-Rabies IgG ELISA Kit

Sample

Serum, plasma or other biological fluids

Species Reactivity

Monkey

Detection Method

iELISA

Intended Use

The Monkey Anti-Rabies IgG ELISA Kit detects and quantifies rabies virus -specific IgG in monkey serum or plasma of vaccinated, immunized and/or infected hosts. This immunoassay is suitable for:
1. Determining immune status relative to non-immune controls;
2. Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization and timing;
3. Qualifying and standardizing vaccine batches & protocols.

Storage

The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label.

Performance Characteristics

Calibrator Values
The Calibrators are dilutions of anti-rabies antibody. Values are assigned as arbitrary anti-rabies virus activity units (see Limits of the Assay).

Sensitivity

The rabies-coated plate and anti-Monkey IgG HRP concentration are optimized to differentiate anti-rabies IgG from background (non-antibody) signal with monkey serum samples diluted 1:100.

General Description

Rabies is a viral disease (Lyssavirus; ssRNA) that causes acute encephalitis (inflammation of the brain) in warm-blooded animals. It is transmitted by animals, most commonly by a bite from an infected animal, and is almost invariably fatal if post-exposure prophylaxis is not administered prior to the onset of severe symptoms.
Humans and animals have been protected, and the disease eradicated in certain geographical regions, by vaccination. Most vaccines have used whole inactivated virus that has been grown in a variety of cell types; vaccines using recombinant proteins of the rabies virus are also available. For rabies control of wildlife, vaccines in bait have proven effective. Improvement of the efficacy of vaccines is an active area of investigation.
The anti-Rabies ELISA is designed with high sensitivity for discriminating lower level antibodies, with specially formulated diluents to minimize interfering background signals.