Rabbit Anti-Rabies NP IgG ELISA Kit

Sample

Serum/Plasma/Biological Samples

Species Reactivity

Rabbit

Detection Method

iELISA

Intended Use

The Rabbit anti-Rabies Virus NP (Nucleoprotein) IgG ELISA Kit is an immunoassay suitable for quantifying IgG antibody activity specific for Rabies NP in serum or plasma. This immunoassay is suitable for:
1. Determining immune status relative to non-immune controls
2. Assessing efficacy of vaccines, including dosage, adjuvantcy, route of immunization, and timing
3. Qualifying and standardizing vaccine batches & protocols
Reagents contain no virus or viral antigens. These kits are designed for studying efficacy of existing vaccines and preparations of more effective rabies vaccine formulations.The assay is for research use only (RUO).

Storage

The microtiter well plate and all other reagents, if unopened, are stable at 2-8°C until the expiration date printed on the box label.

Sensitivity

The Rabies NP antigen coating level, Low NSB diluent, and HRP conjugate concentration are optimized to differentiate anti Rabies NP IgG from background (non-antibody) signal with rabbit serum or plasma samples diluted 1:100.

General Description

Rabies is a fatal zoonotic disease of serious public health concern. The rabies virus is a member of the Lyssavirus genus which have helical symmetry. The lipoprotein envelope carries knob-like spikes composed of Glycoprotein G (VRG). Beneath the envelope is the membrane or matrix (M) protein layer. The core of the virion consists of helically arranged ribonucleoprotein (RV-NP). Old vaccines are made from whole inactivated virus. New recombinant subunit vaccines are based upon purified proteins (RV-NP or VRG) that invoke strong antibodies against the rabies virus. The V-RG vaccine (Raboral/Merial) is harmless to humans and safe for various species of domestic or wild animals.