Porcine Fibrinogen ELISA Kit

Name: Porcine Fibrinogen ELISA Kit
SKU: DEIA9685
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

plasma, serum

Species Reactivity

Pig

Detection Method

Sandwich ELISA

Intended Use

The Porcine Fibrinogen ELISA Kit is intended for the quantitative determination of total fibrinogen antigen in porcine plasma and serum.

Contents of Kit

1. 96-well microtiter strip plate: 8 x 12 well removable strips containing affinity purified anti-porcine fibrinogen antibody dried and blocked on the surface
2. 5X Diluent: 1 bottle of 50 mL; bring to 1 x using DI water
3. 10X Wash Buffer: 1 bottle of 50 mL; bring to 1 x using DI water
4. Porcine fibrinogen Standard: 1 vial of lyophilized Standard
5. Anti-porcine fibrinogen primary antibody: 1 vial of lyophilized biotinylated antibody
6. Peroxidase conjugated avidin: 1 vial of concentrated HRP-labeled avidin
7. TMB substrate solution: 1 bottle of 10 mL solution
8. Stop solution: 1 bottle of 6 mL 1 M sulfuric acid

Storage

All kit components must be stored at 4°C. Store unopened plate and any unused microtiter strips in the pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. DO NOT
freeze/thaw the standards and primary antibody more than once. All other unused kit components must be stored at 4°C. Kit should be used no later than the expiration date.

Detection Range

The assay measures total porcine fibrinogen in the 1.56-800 ng/mL range.

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Mechanism of fine granular area and white etching crack formation in AISI 52100 bearing steel

THEORETICAL AND APPLIED FRACTURE MECHANICS

Authors: Averbeck, Stefan; Spriestersbach, Daniel; Kerscher, Eberhard

Abstract

Fine Granular Area (FGA) and White Etching Cracks (WEC) are two types of fatigue damage phenomena which are characterised by the development of cracks accompanied by a local microstructural transformation. The resulting microstructures share many properties, chiefly a strong reduction in grain size. In this study, the thickness of the transformed zones along the crack formed during FGA and WEC experiments is investigated. It is shown that in WEC, similar to earlier results on the FGA, the size of the transformed zones increases with increasing crack length. For further analysis of the more complex WEC case, an approach is presented that uses the results of a critical plane analysis for the calculation of key figures in fracture mechanics. The results are shown to be very similar to those of the FGA. Drawing on these and earlier results, it is proposed that a common mechanism of WEC and FGA formation exists. In addition, some important differences of the two phenomena are explained within the framework of the common mechanism.

Gravity anomalies and lithospheric flexure around the Longmen Shan deduced from combinations of in situ observations and EGM2008 data

EARTH PLANETS AND SPACE

Authors: She, Yawen; Fu, Guangyu; Wang, Zhuohua; Liu, Tai; Xu, Changyi; Jin, Honglin

Abstract

The current work describes the combined data of three field campaigns, spanning 2009-2013. Their joint gravity and GPS observations thoroughly cover the sites of lithospheric flexure between the Sichuan Basin and the Eastern Tibetan Plateau. The study area's free-air gravity anomalies (FGAs) are updated by using a remove-and-restore algorithm which merges EGM2008 data with in situ observations. These new FGAs show pairs of positive and negative anomalies along the eastern edges of the Tibetan Plateau. The FGAs are used to calculate effective elastic thickness (T-e) and load ratios (F) of the lithosphere. Admittance analysis indicates the Te of Longmen Shan (LMS) to be 6 km, and profile analysis indicates that the T-e of the Sichuan Basin excesses 30 km. The load ratio (F-1 = 1) confirms that the lithospheric flexure of the LMS area can be attributed solely to the surface load of the crust.