Murine Fibrinogen Matched Antibody Pair (ABPR-L003)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
Sufficient reagent for 4 x 96 well plates
Sample
Plasma
Species Reactivity
Murine
Intended Use
This antibody pair set comes with matched antibody pair to detect and quantify protein level of Murine FGA
Contents of Kit
1. Capture Antibody (yellow): 0.4 ml of affinity-purified polyclonal anti-fibrinogen antibody for coating plates.
2. Detecting Antibody (red): 0.4 ml of peroxidase conjugated polyclonal anti-fibrinogen antibody for detection of captured fibrinogen.
Note: Reagents are sufficient for at least 4×96 well plates using recommended protocols. Antibodies are supplied in a 50% (v/v) glycerol solution for storage at –10 to -20°C. Keep vials tightly capped. Do not store in frost-free freezers.
Storage
-10 to -20°C
General Description
Fibrinogen is an abundant plasma protein (5-10 uM) produced in the liver. The intact protein has a molecular weight of 340 kDa and is composed of 3 pairs of disulphide-bound polypeptide chains named Aα, Bβ and γ. Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains. Proteolysis by thrombin results in release of Fibrinopeptide A (FPA, Aα1-16) followed by Fibrinopeptide B (FPB, Bβ1-14) and the fibrin monomers that result polymerize in a half-overlap fashion to form insoluble fibrin fibrils. The chains of fibrin are referred to as α, β and γ, due to the removal of FPA and FPB. The polymerised fibrin is subsequently stabilized by the transglutaminase activated Factor XIII that forms amide linkages between γ chains and, to a lesser extent, α chains of the fibrin molecules. Proteolysis of fibrinogen by plasmin initially liberates C-terminal residues from the Aα chain to produce fragment X (intact D-E-D, which is still clottable). Fragment X is further degraded to non-clottable fragments Y (D-E) and D. Fragment Y can be digested into its constituent D and E fragments. Digestion of non-crosslinked fibrin with plasmin is very similar to the digestion of fibrinogen, which results in production of fragments D and E. Degradation of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the γ chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD. For human crosslinked fibrin, the relative weights of the cleavage fragments produced are: 184 kDa for fragment DD, 92 kDa for D, 50 kDa for E, 1.54 kDa for FPA and 1.57 kDa for FPB.

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References


Genetic variability and forensic efficiency of 39 microsatellite loci in the Li ethnic group from Hainan Island in the South China Sea

ANNALS OF HUMAN BIOLOGY

Authors: Chen, Jing; Xie, Bingbing; Yang, Yaran; Yang, Meng; Liu, Chao; Lv, Yuexin; Chen, Chuguang; Liu, Xu; Fang, Xiangdong; Wu, Huijuan; Yan, Jiangwei

Background: Investigation of allele and genotype frequencies of microsatellite loci in various populations is an essential pre-requisite in forensic application. Aim: The present study obtained population genetic data and forensic parameters of 39 autosomal Short Tandem Repeats (STRs) loci from a Chinese Li ethnic group and estimated the genetic relationships between Li and other reference populations. Subjects and methods: Thirty-nine STR loci, which include D19S433, D5S818, D21S11, D18S51, D6S1043, D3S1358, D13S317, D7S820, D16S539, CSF1PO, Penta D, D2S441, vWA, D8S1179, TPOX, Penta E, TH01, D12S391, D2S1338, FGA, D6S477, D18S535, D19S253, D15S659, D11S2368, D20S470, D1S1656, D22-GATA198B05, D8S1132, D4S2366, D21S1270, D13S325, D9S925, D3S3045, D14S608, D10S1435, D7S3048, D17S1290 and D5S2500, were amplified in two multiplex DNA-STR fluorescence detection systems for 189 unrelated healthy individuals of the Chinese Li ethnic group. The allele frequency distribution and several parameters commonly used in forensic science were statistically analysed. Results: A total of 378 alleles were observed with corresponding allelic frequencies ranging from 0.0026-0.5899. The power of discrimination and power of exclusion ranged from 0.7569-0.9672 and 0.2513-0.7355, respectively. The power of exclusion (PE) ranged from 0.2580-0.7943 for trio paternity cases and 0.1693-0.5940 for duo paternity cases. The polymorphism information content (PIC) ranged from 0.5001-0.8611. The cumulative match probability across these 39 loci was 2.4242x10(-38). Conclusion: The results indicate that 39 STR loci are polymorphic among the Li ethnic group in Hainan Island in the South China Sea. This set of polymorphic STR loci provide highly polymorphic information and forensic efficiency for forensic individual identification and paternity testing, as well as basic population data for population genetics and anthropological research.

FGA formation mechanism for X10CrNiMoV12-2-2 and 34CrNiMo6 for constant and variable amplitude tests under the influence of applied mean loads

FATIGUE & FRACTURE OF ENGINEERING MATERIALS & STRUCTURES

Authors: Ritz, F.; Staecker, C.; Beck, T.; Sander, M.

The aim of the present work is to clarify the fine granular area (FGA) formation mechanism in two steels (tempered 34CrNiMo6 and X10CrNiMoV12-2-2) causing grain refinement in the early state of fatigue for internal crack initiation and propagation in the very high cycle fatigue regime at pure tension-compression loading (R=-1) and for applied mean stresses (R-1). Fatigue tests were performed with constant and variable amplitude at several R values using ultrasonic fatigue testing setups. Failed specimens were investigated using high-resolution scanning electron microscopy and focused ion beam technique with special attention paid to the crack origin and the surrounding microstructure. To prove models for FGA formation proposed in literature, a numerical model to evaluate effective R values and contact stresses between the fracture surfaces depending on the crack length has been realised. The aim of these investigations is to estimate the influence of crack closure effects on FGA formation. FGA formation due to repeating contact of the fracture surfaces according to the model postulated by Hong et al correlates well with the findings for numerical simulations.

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