Human Fibrinogen Matched Antibody Pair (ABPR-L001)

Regulatory status: For research use only, not for use in diagnostic procedures.

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Size
Sufficient reagent for 5 x 96 well plates
Sample
Plasma
Species Reactivity
Human
Intended Use
This antibody pair set comes with matched antibody pair to detect and quantify protein level of Human FGA
Contents of Kit
1. Capture antibody (yellow):0.5 ml of affinity-purified polyclonal anti-fibrinogen antibody for coating plates.
2. Detecting antibody (red):0.5 ml of affinity-purified peroxidase conjugated polyclonal anti-fibrinogen antibody for detection of captured fibrinogen.
Note: Reagents are sufficient for at least 5×96 well plates using recommended protocols. Antibodies are supplied in a 50% (v/v) glycerol solution for storage at –10 to -20°C. Keep vials tightly capped. Do not store in frost-free freezers.
Storage
-10 to -20°C
General Description
Fibrinogen is an abundant plasma protein (5-10 uM) produced in the liver. The intact protein has a molecular weight of 340 kDa and is composed of 3 pairs of disulphide-bound polypeptide chains named Aα, Bβ and γ. Fibrinogen is a triglobular protein consisting of a central E domain and terminal D domains. Proteolysis by thrombin results in release of Fibrinopeptide A (FPA, Aα1-16) followed by Fibrinopeptide B (FPB, Bβ1-14) and the fibrin monomers that result polymerize in a half-overlap fashion to form insoluble fibrin fibrils. The chains of fibrin are referred to as α, β and γ, due to the removal of FPA and FPB. The polymerised fibrin is subsequently stabilized by the transglutaminase activated Factor XIII that forms amide linkages between γ chains and, to a lesser extent, α chains of the fibrin molecules. Proteolysis of fibrinogen by plasmin initially liberates C-terminal residues from the Aα chain to produce fragment X (intact D-E-D, which is still clottable). Fragment X is further degraded to non-clottable fragments Y (D-E) and D. Fragment Y can be digested into its constituent D and E fragments. Digestion of non-crosslinked fibrin with plasmin is very similar to the digestion of fibrinogen, which results in production of fragments D and E. Degradation of crosslinked fibrin by plasmin results in fragment DD (D-Dimer consisting of the D domains of 2 fibrin molecules crosslinked via the γ chains), fragment E (central E domain) as well as DDE in which fragment E is non-covalently associated with DD. For human crosslinked fibrin, the relative weights of the cleavage fragments produced are: 184 kDa for fragment DD, 92 kDa for D, 50 kDa for E, 1.54 kDa for FPA and 1.57 kDa for FPB.

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References


Allele frequency data of 20 STR loci in 2000 Korean individuals

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES

Authors: Kim, Sungmin; Park, Hyun-Chul; Kim, Jong-Sik; Nam, Younhyong; Kim, Hye Yeon; Park, Jihye; Chung, Ukhee; Lee, Jinmyung; Lim, Si-Keun; Park, Su-Jeong

To increase the discrimination power of human identification in forensic and to evaluate expanded short tandem repeat (STR) loci, we examined a Korean sample population of 2000 unrelated individuals using both GlobalFiler (TM) PCR Amplification kit and PowerPlex (R) Fusion System. Allele frequencies for the 20 STR loci (D3S1358, vWA, D16S539, CSF1PO, TPOX, D8S1179, D21S11, D18S51, D2S441, D19S433, TH01, FGA, D22S1045, D5S818, D13S317, D7S820, D10S1248, D1S1656, D12S391, and D2S1338) were calculated. Allele frequencies of the 20 STR loci would be useful for personal identification and paternity testing in forensics and as a standard reference database in Korea.

Evaluation of the genetic parameters and mutation analysis of 22 STR loci in the central Chinese Han population

INTERNATIONAL JOURNAL OF LEGAL MEDICINE

Authors: Wang Hongdan; Kang Bing; Su Ning; He Miao; Zhang Bo; Guo Yuxin; Zhu Bofeng; Liao Shixiu; Zeng Zhaoshu

At present, the Han nationality is China's main ethnic group and also the most populous nation in the world. This is a great resource to study microsatellite mutations and for the study of ethnogeny. The aim of this study is to investigate the genetic polymorphisms and mutations of 22 autosomal STR loci in 2475 individuals from Henan province, China. DNA is amplified and genotyped using PowerPlex (TM) 24 system. The gene frequencies, forensic parameters, and the mutation rate of the 22 STR loci are analyzed. A total of 295 alleles are observed in this Henan Han population, and the allelic frequencies ranged from 0.0003 to 0.5036. In order to investigate the genetic relationships between the Henan Han and the other 14 different populations, our present data were compared with previously published data for the same 15 STR loci. The results indicated that the Henan Han had closer genetic relationships the groups including Minnan Han, Maonan, Yi and Guangdong Han groups while the South morocco population, the Moroccan population, the Malay group, and the Uigur stand away from Henan Han. Except of D2S441, D13S317, PentaE, D2S1338, D5S818, TPOX and D19S433, the mutation events are found in the other 15 STR loci. A total of 40 mutation events are observed in the 15 STR loci. The mutation rates are ranged from 0 to 4.85 x 10(-3). In this study, 39 mutations are single-step mutations, and only one at FGA comprised two steps. STR mutation is commonly existed in paternity testing, while there are no STR mutation studies of the 22 STR loci in the Henan Han population. It is of great importance in forensic individual discrimination and paternal testing.

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