Human SPARC ELISA Kit

Sample

serum, plasma, urine, tissues homogenates, cell culture supernatants, other biological fluids

Species Reactivity

Human

Detection Method

Sandwich ELISA

Intended Use

This immunoassay kit allows for the in quantitative determination of Human Osteonectin, ON concentrations in serum, plasma, urine, tissue homogenates and cell culture supernates and other biological fluids.

Contents of Kit

1. Assay Plate 1
2. Standard 2
3. Sample Diluent 1 x 20 mL
4. Assay Diluent A 1 x 10 mL
5. Assay Diluent B 1 x 10 mL
6. Detection Reagent A 1 x 120 μL
7. Detection Reagent B 1 x 120 μL
8. Wash Buffer (25X) 1 x 30 mL
9. Substrate 1 x 10 mL
10. Stop Solution 1 x 10 mL
11. Plate sealer for 96 wells 5

Storage

The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit, substrate should be always stored at 4°C. Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. For more detailed information, please download the following document on our website.

Detection Range

3.12 ng/mL-200 ng/mL