Human P62/SQSTM1 (Sequestosome-1) ELISA Kit

Sample

Serum, plasma, cell culture supernatant and other biological samples

Species Reactivity

Human

Detection Method

sELISA

Intended Use

In vitro quantitative determination of SQSTM1 concentrations in serum, plasma, cell culture supernatant and other biological samples.

Contents of Kit

The sealed kit can be stored at 2-8°C. The storage condition for opened kit is specified in the table below:
1. ELISA Microplate(Dismountable), Size(96T): 8×12. Storage Condition for Opened Kit: Put the rest strips into a sealed foil bag with the desiccant. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
2. Lyophilized Standard, Size(96T): 2vial. Storage Condition for Opened Kit: Put the rest standards into a desiccant bag. Stored for 1 month at 2-8°C; Stored for 12 month at -20°C
3. Biotin-labeled Antibody(Concentrated, 100X), Size(96T): 120ul. Storage Condition for Opened Kit: 2-8°C (Avoid Direct Light)
4. HRP-Streptavidin Conjugate(SABC, 100X), Size(96T): 120ul. Storage Condition for Opened Kit: 2-8°C (Avoid Direct Light)
5. TMB Substrate, Size(96T): 10ml. Storage Condition for Opened Kit: 2-8°C (Avoid Direct Light)
6. Sample Dilution Buffer, Size(96T): 20ml. Storage Condition for Opened Kit: 2-8°C
7. Antibody Dilution Buffer, Size(96T): 10ml. Storage Condition for Opened Kit: 2-8°C
8. SABC Dilution Buffer, Size(96T): 10ml. Storage Condition for Opened Kit: 2-8°C
9. Stop Solution, Size(96T): 10ml. Storage Condition for Opened Kit: 2-8°C
10. Wash Buffer(25X), Size(96T): 30ml. Storage Condition for Opened Kit: 2-8°C
11. Plate Sealer, Size(96T): 5 pieces
12. Product Description, Size(96T): 1 copy
Note: The liquid reagent bottle contains slightly more reagent than indicated on the label. Please use pipette accurately measure and do proportional dilution.

Storage

2-8°C (for sealed box), please do not freeze! See kit label for expiry date

Detection Range

0.156-10ng/ml

Sensitivity

0.094ng/ml

General Description

Uniprot ID:Q13501
SQSTM1 (Sequestrome-1), also called p62, is a widely expressed, stress-inducible, multifunctional 62 kDa intracellular protein. SQSTM1 contains multiple adaptor domains that allow interaction with proteins in NGF/NFkB and other signaling pathways (notably TRAF6, atypical protein kinase C family and Src family), polyubiquitin, proteasome subunits and many others. SQSTM1 shuttles ubiquitinylated proteins to the proteasome and is important in autophagy and apoptosis. Its dysregulation is associated with Paget's disease of bone, Parkinson's and Alzheimer's diseases, and cancers.