Anti-Human SQSTM1 monoclonal antibody (DCABH-169)

Rabbit Anti-Human SQSTM1 monoclonal antibody for ELISA, ICC, IF, WB

Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
22ID25MD36
Species Reactivity
Human
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution:
ELISA: Assay-Dependent
ICC: 1 µg/ml
IF: 1 µg/ml
WB: 0.5 µg/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Western blot analysis of Sequestosome-1 recombinant protein (lane 1, 200ng) and endogenous Sequestome-1 in extracts from HeLa cells treated with 60 mM chloroquinone for 16 hours (lane 2) using a Sequestome-1 recombinant rabbit oligoclonal antibody at a concentration of 1 µg/mL. A rabbit anti-tubulin antibody was blotted as a loading control and the blots were developed using chemiluminescence (ECL) method with a goat anti-rabbit HRP-conjugated secondary antibody. Results show a band at ~62kDa.

Target


Alternative Names
SQSTM1; sequestosome 1; p60; p62; A170; OSIL

Citations


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Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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References


Sakai, K; Fukuda, T; et al. Beading of the astrocytic processes (clasmatodendrosis) following head trauma is associated with protein degradation pathways. BRAIN INJURY 27:1692-1697(2013).
Lao, YZ; Wan, G; et al. The natural compound oblongifolin C inhibits autophagic flux and enhances antitumor efficacy of nutrient deprivation. AUTOPHAGY 10:736-749(2014).

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