Anti-Human SQSTM1 monoclonal antibody (DCABH-169)

Rabbit Anti-Human SQSTM1 monoclonal antibody for ELISA, ICC, IF, WB Datasheet

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Specifications


Host Species
Rabbit
Antibody Isotype
IgG
Clone
22ID25MD36
Species Reactivity
Human
Conjugate
Unconjugated

Applications


Application Notes
Recommended dilution:
ELISA: Assay-Dependent
ICC: 1 µg/ml
IF: 1 µg/ml
WB: 0.5 µg/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.

Data Examples


Western blot analysis of Sequestosome-1 recombinant protein (lane 1, 200ng) and endogenous Sequestome-1 in extracts from HeLa cells treated with 60 mM chloroquinone for 16 hours (lane 2) using a Sequestome-1 recombinant rabbit oligoclonal antibody at a concentration of 1 µg/mL. A rabbit anti-tubulin antibody was blotted as a loading control and the blots were developed using chemiluminescence (ECL) method with a goat anti-rabbit HRP-conjugated secondary antibody. Results show a band at ~62kDa.

Target


Alternative Names
SQSTM1; sequestosome 1; p60; p62; A170; OSIL

Citations


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References


Nogalska, A; Terracciano, C; et al. p62/SQSTM1 is overexpressed and prominently accumulated in inclusions of sporadic inclusion-body myositis muscle fibers, and can help differentiating it from polymyositis and dermatomyositis. ACTA NEUROPATHOLOGICA 118:407-413(2009).
Shen, CC; Wang, WZ; et al. Chloroquine blocks the autophagic process in cisplatin-resistant osteosarcoma cells by regulating the expression of p62/SQSTM1. INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE 32:448-456(2013).

Custom Antibody Labeling


We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody. Learn More

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