Anti-Human SQSTM1 monoclonal antibody (DCABH-169)
Rabbit Anti-Human SQSTM1 monoclonal antibody for ELISA, ICC, IF, WB
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ICC: 1 µg/ml
IF: 1 µg/ml
WB: 0.5 µg/ml
*Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own experiment using appropriate negative and positive controls.
Western blot analysis of Sequestosome-1 recombinant protein (lane 1, 200ng) and endogenous Sequestome-1 in extracts from HeLa cells treated with 60 mM chloroquinone for 16 hours (lane 2) using a Sequestome-1 recombinant rabbit oligoclonal antibody at a concentration of 1 µg/mL. A rabbit anti-tubulin antibody was blotted as a loading control and the blots were developed using chemiluminescence (ECL) method with a goat anti-rabbit HRP-conjugated secondary antibody. Results show a band at ~62kDa.
SQSTM1; sequestosome 1; p60; p62; A170; OSIL
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Bjorkoy, G; Lamark, T; et al. MONITORING AUTOPHAGIC DEGRADATION OF P62/SQSTM1. METHODS IN ENZYMOLOGY: AUTOPHAGY IN MAMMALIAN SYSTEMS, VOL 452, PT B 452:181-197(2009).
Tanji, K; Maruyama, A; et al. Keap1 Is Localized in Neuronal and Glial Cytoplasmic Inclusions in Various Neurodegenerative Diseases. JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY 72:18-28(2013).
Custom Antibody Labeling
We offer labeled antibodies using our catalogue antibody products and a broad range of intensely fluorescent dyes and labels including HRP, biotin, ALP, Alexa Fluor® dyes, DyLight® Fluor dyes, R-phycoerythrin (R-PE), at scales from less than 100 μg up to 1 g of IgG antibody.