Animals were immunized with human cytolytic T lymphocytes. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody will inhibit HLA DR mediated T cell cytotoxicity. The depositor states that the cell line should not be maintained in culture continuously for greater than four months or antibody production may be lost. Cloning and freezing of the cells after minimal passage (with selection of positive clones) will prevent loss of antibody producing cells. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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