Cytotoxic T cells from C57BL/6 mice immunized with P815 cells
Fusion Species
Rat X Mouse Heterohybridoma
Immunological Donor
Rat spleen
Myeloma
NS-1
Clone
N28/5.5.22.0
Cell Line Description
Animals were immunized with cytotoxic T cells from C57BL/6 mice immunized with P815 cells. Spleen cells were fused with NS-1 myeloma cells. Monoclonal antibodies against LFA-1 can block killing by inhibiting the adhesion between cytolytic T cells and target cells.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 3-4 days, start cells at 5x10^4 cells/mL and maintain cultures between 5x10^4-5x10^5 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
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