GeneralInformation
Immunogen
An ebv-transformed b cell line from a patient with mhc class ii deficiency
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Described as secreting a mouse monoclonal antibody (igg2b) CD71; the cell line was established in 1986 by fusion of PX63AG8.653 cells with spleen cells of a mouse immunized with an EBV-transformed B cell line from a patient with MHC class II deficiency . .
Viruses: ELISA: reverse transcriptase negative; PCR: SMRV
Morphology
adherent cells growing as monolayers, some cells grow in suspension
Growth Properties
monolayers, some cells grow in suspension
CultureMethod
Subculturing
Split ratio: 1: 3, every 2 days; seed out at 0.2 x 10^6 cells/ml; Maintain at 0.2-0.4 x 10^6 cells/ml; Doubling time: about 20-30 hours; Harvest: maximal density of about 5 x 10^6 cells/25 cm2.
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium(20%FBS), add 10%(v/v) DMSO
Target
Synonyms
2610028K12Rik; AI195355; AI426448; AU015758; CD71; CD71 antigen; E430033M20Rik; Mtvr-1; Mtvr1; p90; sTfR; T9; TFR; TFR1; TFRC; TFRC transferrin receptor (p90 CD71); TR; transferrin receptor; transferrin receptor protein 1; Transferrin receptor protein 1, serum form; TRFR;
Citations
Publication ()
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COA
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