Animals were immunized with the mouse erythroleukemia line 745.6. Spleen cells were fused with S194/5.XXO.BU.1 myeloma cells. The antibody does not prevent cell proliferation. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 4x10^4 cells/mL and maintain cultures at or below 4x10^5 cells/ml
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