Animals were immunized with the HL-60 promyelocytic leukemia cell line. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody precipitates an 87000 dalton glycoprotein present on the surface of K562 leukemia cells. The antibody reacts strongly with all morphologically recognizable precursors of the human erythroid lineage. Tested and found negative for ectromelia virus (mousepox).
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 1x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase. Freezing medium: to complete growth medium , add 10% (v/v) DMSO
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