Recombinant gp120 (rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB, SF2, and Z6)
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
66-94
Cell Line Description
Animals were sequentially immunized with recombinant gp120 (rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB, SF2, and Z6). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. 55-83 is one of a panel of antibodies developed by Dr. Suzanne Epstein that reacts with the gp120 of HIV-1. In ELISA the antibody reacts strongly with rgp120 IIIB and with gp120 MN (an isolate not used during immunization). It binds to the C1 region (positions 88 - 98) gp120 synthetic peptide used to map antibody reactivity.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
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