Recombinant gp120 (rgp120) from a single isolate of HIV-1 (IIIB)
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0-Ag14
Clone
57-3
Cell Line Description
Animals were repeatedly immunized with recombinant gp120 (rgp120) from a single isolate of HIV-1 (IIIB). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. In ELISA the antibody reacts strongly with rgp120 IIIB, but does not react with gp120 MN.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
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