Mouse Anti-Human HIV-1 gp120 Hybridoma [66-47]

Immunogen

Recombinant gp120 (rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB, SF2, and Z6)

Fusion Species

Mouse X Mouse Hybridoma

Immunological Donor

Mouse spleen

Myeloma

Sp2/0-Ag14

Clone

66-47

Cell Line Description

Animals were sequentially immunized with recombinant gp120 (rgp120) from three different nonglycosylated isolates of HIV-1 (IIIB, SF2, and Z6). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. In ELISA the antibody reacts strongly with rgp120 IIIB. In immunofluorescence assays, it binds weakly to cell surface expressed gp160 and also shows weak cross-reactivity by ELISA with gp120 MN (an isolate not used during immunization). The antibody does not bind to the gp120 synthetic peptides that were used to map antibody reactivity and was not able to cross block any other antibodies in the panel.

Morphology

Lymphoblast

Growth Properties

Suspension

Subculturing

Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml

Storage

Liquid nitrogen vapor phase.