Recombinant human follicle stimulating hormone (FSH) receptor expressed in E. coli
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Myeloma
Sp2/0
Clone
GTIS-434
Cell Line Description
Animals were immunized with recombinant human follicle stimulating hormone (FSH) receptor expressed in E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the N-terminal extracellular domain (amino acids domain 23-358) of the human follicle stimulating hormone (FSH) (hormone binding unit).
Application Notes
WB, ELISA, ICC, and IHC of the human FSH receptor.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
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