GeneralInformation
Immunogen
Ecombinant human follicle stimulating hormone (FSH) receptor expressed in E. coli
Fusion Species
Mouse X Mouse Hybridoma
Immunological Donor
Mouse spleen
Cell Line Description
Animals were immunized with recombinant human follicle stimulating hormone (FSH) receptor expressed in E. coli. Spleen cells were fused with Sp2/0 mouse myeloma cells. The antibody recognizes the N-terminal extracellular domain (amino acids domain 23-358) of the human follicle stimulating hormone (FSH) (hormone binding unit).
Application Notes
WB, ELISA, ICC, and IHC of the human FSH receptor.
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 2x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium(20% horse serum) , add 7.5% (v/v) DMSO
Target
Synonyms
FSHR; follicle stimulating hormone receptor; ODG1; follicle-stimulating hormone receptor; FSHRO; LGR1; FSH receptor; follitropin receptor; MGC141667; MGC141668;
Citations
Publication ()
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COA
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