Animals were immunized with LPS activated DBA/2J mouse B cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts with the B7-2 (GL1) antigen present on activated mouse B cells.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Freezing medium: to complete growth medium, add 5%(v/v) DMSO
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