Anti-Carazolol polyclonal antibody

A rapid and sensitive multi-residue method was developed to attempt to confirm the presence of the beta-blocker carazolol and the tranquillizers acepromazine, azaperone, chlorpromazine, propionylpromazine and xylazine in pig muscle tissues. The procedure involves determination by liquid chromatography coupled with tandem mass spectrometry. The liquid chromatographic separation was performed on a Symmetry C-18 column with gradient elution, A mixture of aqueous buffer, containing 0.01% m/v trifluoroacetic acid (pH 3.5), and acetonitrile at a flow rate of 0.4 ml min(-1) was used as the mobile phase, The abundant parent ions [M + H+] produced by positive electrospray ionisation were selected for collisional dissociation with argon. Fragment ions were recorded with daughter ion scan and multiple reaction monitoring. The analytes were identified unambiguously by assessing retention times and diagnostic ions in meat samples spiked from 50 mu g kg(-1) [maximum residue limit (MRL) for azaperone and azaperol] to 5 mu g kg(-1) (MRL for carazolol).

Carazolol [4-(2-hydroxy-3-isopropyl-amino-propoxy)-carbazole], a beta(3)-adrenoceptor agonist, is clinically used in the treatment of hypertension, cardiac arrhythmias and angina pectoris. Despite the beneficial effect of the drug, its high dose may contribute to cardiotoxicity. This study was conducted to examine whether carazolol can influence hydroxyl radical formation by a Fenton-like reaction [Co(II) + H2O2 + HO-] in the presence of ethylenediaminetetraacetic acid. The oxygen free radicals and singlet oxygen (O-1(2)) formation was traced by three different assay methods: chemiluminescence (CL), an electron spin resonance (ESR) spin trapping with 2,2,6,6-tetramethyl-4-piperidine and 5,5-dimethyl-1-pyrroline-1-oxide, and spectrophotometric determination of O-1(2) based on bleaching of p-nitrosodimethylaniline. The effect of hydroxyl radical inhibitors and O-1(2) quenchers on peroxidation of carazolol was also examined. The results indicated that carazolol enhanced the HO radical and O-1(2) formation in a Fenton-like reaction. Copyright (C) 2010 John Wiley & Sons, Ltd.