Animals were immunized with a rough strain of 0157:H7 (E. coli HA1, deficient in the 0157 antigen). Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for E coli 0157:H7 and 026:H11 both of which are enterohemorrhagic. It reacts with a protein antigen of 13000 daltons.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 2x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
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