E.coli O157 ELISAKit

Sample

ground beef, milk, lettuce, etc

Species Reactivity

Human

Detection Method

sELISA

Intended Use

CD E. coli O157 ELISA Kit is an enzyme immunoassay developed for detection and quantitation of the E. coli O157. The ELISA antibodies specifically recognize the E.coli O157 strain, will not react with other E. coli strains with different O antigens and other types of bacteria such as Shigella sonnei, Pseudomonas aeruginosa and Salmonella flexneri. The ELISA kit has a detection sensitivity limit of 1.2 × 10⁴ CFU equivalents/mL E. coli O157. Each kit provides sufficient reagents to perform up to 96 assays including standard curve and food test samples.

Contents of Kit

Box 1 (shipped at room temperature)
1. Anti- E.coli O157 Antibody Coated Plate: One strip well 96-well plate.
2. FITC-Conjugated Anti- E.coli O157 Monoclonal Antibody: One 20 μL vial.
3. HRP-Conjugated Anti-FITC Monoclonal Antibody: One 20 μL vial.
4. Assay Diluent: One 50 mL bottle.
5. 10× Wash Buffer: One 100 mL bottle.
6. Substrate Solution: One 12 mL amber bottle.
7. Stop Solution: One 12 mL bottle.
Box 2 (shipped on blue ice packs)
1. Heat Killed E.coli O157 Standard: One 200 μL vial of 5 × 10⁸ CFU equivalents/mL heat killed E.coli O157 in LB broth with 30% Glycerol.
Note: Before heat inactivation (90-100 °C for 30 min), the colony forming units (CFUs) of E.coli O157 is predetermined by serial dilution and culture on LB agar plates.

Storage

Upon receiving, aliquot and store heat killed E.coli O157 Standard at -80 °C and avoid freeze/thaw. Store all other components at 4°C.

General Description

E. coli is one of the many groups of bacteria that live in the intestines of healthy humans and most warm-blooded animals. E. coli's serotyping is based on the flagellar (H) and polysaccharide (O) antigens.
Shiga toxin–producing E.coli strains are major foodborne pathogens and have caused a large number of human illnesses. E.coli O157:H7 is the most well-known serotype and it can cause diarrhea, hemorrhagic colitis, and in more severe cases hemolytic uremic syndrome. Most E.coli O157:H7 infection outbreaks have been linked to the consumption of ground beef.

Standard Curve

The following figures demonstrate typical E.coli O157 ELISA results. One should use the data below for reference only. This data should not be used to interpret actual results.
Figure 1: Heat Killed E.coli O157 ELISA Standard Curve.

Figure 2: E.coli O157 ELISA Selectivity. Heat killed 5×10⁸ equivalent CFUs/mL bacterial culture was diluted in Assay Diluent and subjected to E.coli O157 ELISA Kit according to Assay Protocol.

Table 2. E.coli O157 ELISA Specificities.