Animals were immunized with whole bacterium antigen from E. coli O157:H7 . Spleen cells were fused with P3X63Ag8.653 mouse myeloma cells. The antibody is specific for E. coli O157 O-antigen and for the Salmonella serogroup N O-antigen (which is identical to the E. coli O157 antigen). High titer ascites can be produced but these cells become lethal to mice within approximately 5 days post-intraperitioneal injection.
Application Notes
ELISA, WB, lateral flow and chemiluminescense assays.
Morphology
Lymphoblast
Growth Properties
Suspension
CultureMethod
Subculturing
Incubate cells at 37°C with 5% CO2 in air atmosphere, renew medium every 2-3 days, start cells at 1x10^5 cells/mL and maintain cultures between 1x10^5-1x10^6 cells/ml
Storage
Liquid nitrogen vapor phase.
Citations
Publication ()
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