Sample
Urine, Serum, Plasma, Tissue extracts, Cell lysate, Cell culture media, Other biological fluids
Species Reactivity
Species-independent
Detection Method
Enzymes Assay
Intended Use
Detection and quantification of Alkaline Phosphatase (ALP) activity in urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples.
Contents of Kit
| Component | Quantity | Storage |
| 96-Well Microplate | 1 | |
| Assay Buffer | 30 mL x 4 | 4°C |
| Reaction Buffer | 4 mL x 1 | 4°C, keep in dark |
| Substrate | Powder x 1 | 4°C, keep in dark |
| Dye Reagent I | Powder x 1 | 4°C, keep in dark |
| Dye Reagent II | Powder x 1 | 4°C, keep in dark |
| Standard | Powder x 1 | 4°C |
| Positive Control | 0.1 μL x 1 | 4°C |
| Plate Sealer | 3 | |
| Instruction Manual | 1 | |
Detection Range
0.05 mmol/L - 5 mmol/L
General Description
Alkaline phosphatase (ALP) catalyzes the hydrolysis of phosphate esters in an alkaline environment, resulting in the formation of an organic radical and inorganic phosphate. In mammals, this enzyme is found mainly in the liver and bones. Marked increase in serum ALP levels, a disease known as hyperalkalinephosphatasemia, has been associated with malignant biliary obstruction, primary biliary cirrhosis, primary sclerosing cholangitis, hepatic lymphoma and sarcoidosis. The assay is initiated with the enzymatic hydrolysis of the disodium phenyl phosphate by alkaline phosphatase. The enzyme catalyzed reaction products can be measured at a colorimetric readout at 510 nm.
Citations
Publication ()
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