Sheep Bone Alkaline Phosphatase ELISA Kit

Humans are daily exposed to 7,12-dimethylbenz(a)anthracene (DMBA), a well known polycyclic aromatic hydrocarbons (PAH). This study investigated the role of dietary intake of Vitamin K (VK), a polyphenolic compound, with potential antioxidative properties, against DMBA-induced hepatotoxicity. Sixty experimental animals (120-150 g) were divided into six groups (A-F): Control, DMBA (80 mg/kg bw) only, VK (0.00 g/10 kg) diet only, VK (7.5 g/10 kg) diet only, DMBA + VK (0.0 g/10 kg) diet and DMBA + VK (7.5 g/10 kg) diet. Single oral administration of DMBA (80 mg/kg body weight) to Wistar rats resulted in hepatic damage after 16 weeks. DMBA significantly (P < .05) decreased the activities of catalase (CAT), superoxide dismutase (SOD), glutathione-S-transferase (GST) and glutathione peroxidase (GPx). Levels of reduced glutathione (GSH) and Vitamin C were significantly decreased with increase in malondialdehyde (MDA) and nitric oxide (NO) levels in serum and liver. Aspartate aminotransaminase (AST), alanine aminotransaminase (ALT), gamma-glutamyltransferase (GGT), alkaline phosphatase (ALP), and lactate dehydrogenase (LDH) activities were significantly (P < .05) elevated in the serum but reduced in the liver of DMBA-administered group. Ingestion of 7.5 g/10 kg VK diet prevented the up regulations in inflammatory biomarkers (granulocyte macrophage colony stimulating factor (GM-CSF) and interleukin 17A (IL-17A)) which elicited liver damaged in the DMBA-treated group. DMBA induced hepatic alterations in DMBA-treated group but was restored to near normal in VK (7.5 g/10 kg) diet group. These findings suggest the protective potential of increased dietary intake of vitamin K against DMBA-induced hepatic dysfunction.

In the Argentera-Mercantour Massif, swarms of high-K talc-allcaline lamprophyres intruded into Carboniferous migmatites and early Permian granites, likely in Permian-Triassic times. The dykes collected multiple magmatic injections, the latest of which of alkaline affinity. Syn-intrusive vesicles and straight chilled margins suggest that the lamprophyres emplaced at a shallow crustal level. Lower greenschist facies mineral assemblages replaced lamprophyre igneous minerals as a result of late-intrusive hydrothermal circulation that occurred soon after the dyke emplacement. The hydrothermal event is constrained at T = 300-350 degrees C and P < 0.1 GPa by matching thermobarometry and pseudosection results. During the Alpine collision, the lamprophyres were intersected by upper greenschist fades mylonitic shear zones developed at T = 420-450 degrees C and P = 0.2-0.4 GPa, which are the metamorphic peak conditions of the Argentera-Mercantour Massif in Alpine times. (C) 2020 Elsevier B.V. All rights reserved.