Canine Bone Alkaline Phosphatase ELISA Kit

Sample

Serum, plasma, tissue homogenates and other biological fluids.

Species Reactivity

Canine

Detection Method

cELISA

Intended Use

This Canine Bone Alkaline Phosphatase (BALP) Elisa kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine BALP. This ELISA kit for research use only, notfor therapeutic or diagnostic applications!

Contents of Kit

1. MICROTITER PLATE, 96 wells, strip well
2. ENZYME CONJUGATE, 6 mL 1 vial
3. STANDARD A 0 mU/mL, 1 vial
4. STANDARD B 25 mU/mL, 1 vial
5. STANDARD C 50mU/mL, 1 vial
6. STANDARD D 100 mU/mL, 1 vial
7. STANDARD E 250 mU/mL, 1 vial
8. STANDARD F 500 mU/mL, 1 vial
9. SUBSTRATE A, 6 mL 1 vial
10. SUBSTRATE B, 6 mL 1 vial
11. STOP SOLUTION, 6 mL 1 vial
12. WASH SOLUTION (100×), 10 mL 1 vial
13. BALANCE SOLUTION, 3 mL 1 vial
14. INSTRUCTION, 1
NOTE: The BALANCE SOLUTION is used only when the sample is cell culture supernatants, body fluid and tissue homogenate; if the sample is serum or plasma, then the BALANCE SOLUTION is a superfluous reagent.
The types of sample:
Sample I: serum or plasma
Sample II: cell culture supernatants, body fluid and tissue homogenate

Storage

All reagents provided are stored at 2-8° C. Refer to the expiration date on the label

Precision

In the same lot CV%: 4.4, 5.62)
Different lot CV%: 6.6, 7.9

Sensitivity

The sensitivity in this assay is 1.0 mU/mL.

Standard Curve

For reference only