Mouse Anti-Beta-2-Glycoprotein I IgA ELISA Kit

Sample

Serum, plasma

Species Reactivity

Mouse

Detection Method

iELISA

Intended Use

The Mouse Anti-Beta-2-Glycoprotein I IgA ELISA Kit is designed for the Semi-quantitative analysis of Mouse Serum, plasma samples.

Contents of Kit

1. Polystyrene microwell ELISA plates coated with a purified antigen(12-1 x 8 wells), with holder in foil package containing desiccants
2. Negative Control, 1 vial of buffer 1.2 mL
3. Positive Control, 1 vial of buffer 1.2 mL
4. Calibrator A, 1 vial of buffer containing preservative, prediluted, 1.2 mL
5. Calibrator B, 1 vial of buffer containing preservative, prediluted, 1.2 mL
6. Calibrator C, 1 vial of buffer containing preservative, prediluted, 1.2 mL
7. Calibrator D, 1 vial of buffer containing preservative, prediluted, 1.2 mL
8. Sample Diluent, 1 vial – colored straw containing PBS-buffered saline, protein stabilizers and preservative, 50 mL.
9. Antibody Enzyme Conjugate, colored blue containing buffer, protein stabilizers and preservative, 12 mL
10. Wash Buffer (20 ×), 50 mL
11. TMB Chromogen, containing stabilizers, 10 mL
12. Stop Solution, Colorless, 10 mL

Storage

2–8 °C, protected from light and moisture.

Detection Range

0 - 200 U

General Description

An Anti-Beta2 Glycoprotein I IgG/IgM test detects autoantibodies that attack the protein beta-2 glycoprotein I (β2GPI), which is associated with antiphospholipid syndrome (APS). A positive result may indicate a higher risk of blood clots or recurrent miscarriages, but for a diagnosis of APS, the antibodies must be persistent, detected on two separate occasions at least 12 weeks apart. The test is often ordered with other antiphospholipid antibody tests, such as anticardiolipin antibodies and lupus anticoagulant.