Contents of Kit
1. Capture Antibody (yellow): 0.5 ml of polyclonal affinity-purified anti-β2 GPI antibody for coating plates.
2. Detecting Antibody (red): 0.5 ml of peroxidase-conjugated polyclonal anti-β2 GPI antibody for detection of captured β2GPI.
Note: Reagents are sufficient for at least 5×96 well plates using recommended protocols. Antibodies are supplied in a 50% (v/v) glycerol solution for storage at –10 to -20°C. Keep vials tightly capped. Do not store in frost-free freezers.
Apolipoprotein-H, also known as β2-Glycoprotein I (β2GPI), is a plasma glycoprotein that circulates at a concentration of 200 ug/ml (4 μM). Synthesized in the liver, β2GPI is a single chain molecule of 48 kDa, consisting of five repeating internally disulphide-bonded structures referred to as sushi domains. Relative to other glycoproteins, β2GPI has an unusually high content of cysteine (6.2%), proline (8.3%) and carbohydrate (19%). Almost half the circulating β2GPI in plasma is associated with lipoproteins of all major fractions. β2GPI has been demonstrated to bind negatively charged phospholipids, heparin and platelets. Although the precise function(s) are as yet unknown, β2GPI has been demonstrated to interfere with blood coagulation by competitively binding to negatively charged phospholipid surfaces exposed during cell activation or damage. Recent evidence also implicates β2GPI as a cofactor recognized by anti-phospholipid antibodies present in some autoimmune disorders such as systemic lupus erythematosus (SLE).