Human Anti-Beta2 Glycoprotein I IgG/IgM ELISA Kit

Name: Human Anti-Beta2 Glycoprotein I IgG/IgM ELISA Kit
SKU: DEIA1814
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

COA

SDS

Datasheet

Summary

Sample

Serum, plasma

Species Reactivity

Human

Detection Method

iELISA

Intended Use

Anti-beta-2-Glycoprotein I IgG/lgM is an ELISA test system for the quantitative measurement of IgG and IgM class autoantibodies against beta-2-Glycoprotein I in human serum or plasma. This product is intended for research use only.

Contents of Kit

1. MICROPLATE: 1 One divisible microplate consisting of 12 modules of 8 wells each. Ready to use.
2. CALIBRATOR A: 1×1.5 mL Calibrator A 0 U/mL, containing serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
3. CALIBRATOR B: 1× 1.5 mL Calibrator B 6.3 U/mL, containing beta-2-Glycoprotein I antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use
4. CALIBRATOR C: 1×1.5 mL Calibrator C 12.5 U/mL, containing beta-2-Glycoprotein I antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use.
5. CALIBRATOR D: 1×1.5 mL Calibrator D 25 U/mL, containing beta-2-Glycoprotein I antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use
6. CALIBRATOR E: 1×1.5 mL Calibrator E 50 U/mL, containing beta-2-Glycoprotein Iantibodies in a serum/buffer matrix (PBS, BSA, NaN₃ 0.09%), yellow. Ready to use.
7. CALIBRATOR F: 1×1.5 mL Calibrator F 100 U/mL, containing beta-2-Glycoprotein I antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use
8. CONTROL +: 1×1.5 mL Control positive, containing beta-2-GlycoproteinI antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use. The concentration is specified on the certificate of analysis.
9. CONTROL -: 1×1.5 mL Control negative, containing beta-2-Glycoprotein I antibodies in a serum/buffer matrix (PBS, BSA, detergent, NaN₃ 0.09%), yellow. Ready to use. The concentration is specified on the certificate of analysis.
10. DILUENT: 20 mL Sample Buffer P, containing PBS, BSA, detergent, preservative NaN₃ 0.09%, yellow, concentrate 5×
11. CONJUGATE G: 15 mL Enzyme Conjugate; containing anti-human IgG antibodies, HRP labelled; PBS, BSA, detergent, preservative Proclin 0.05%, light red. Ready to use.
12. CONJUGATE M: 15 mL Enzyme Conjugate; containing anti-human IgM antibodies, HRP labelled; PBS, BSA, detergent, preservative Proclin 0.05%, light red. Ready to use
13. TMB: 15 mL TMB Substrate; containing 3,3',5,5'-Tetramethylbenzidin, colourless. Ready to use.
15. STOP: 15 mL Stop Solution; contains acid. Ready to use.
16. WASH: 20 mL Wash Buffer, containing Tris, detergent, preservative NaN₃0.09%; 50X conc.

Storage

1. Store test kit at 2 °C -8 °C in the dark.
2. Do not expose reagents to heat, sun, or strong light during storage and usage.
3. Store microplate sealed and desiccated in the clip bag provided. Shelf life of the unopened test kit is 18 months from day of production. Unopened reagents are stable until expiration of the kit. See labels for individual batch.
4. Diluted Wash Buffer and Sample Buffer are stable for at least 30 days when stored at 2-8°C. We recommend consumption on the same day.

Precision

Intra-assay precision: Coefficient of variation (CV) was calculated for each of three samples from the results of 24 determinations in a single run. Results for precision-within-assay are shown in the table below.
Inter-assay precision: Coefficient of variation (CV) was calculated for each of three samples from the results of 6 determinations in 5 different runs. Results for run-to-run precision are shown in the table below.

Detection Range

The calculation range of this ELISA assay is IgG: 0-100 U/mL IgM: 0 -100 U/mL

Sensitivity

Functional sensitivity was determined to be: IgG: 0.5 U/mL IgM: 0.5 U/mL

Standard Curve

For quantitative results plot the optical density of each calibrator versus the calibrator concentration to create a calibration curve. The concentration of samples may then be estimated from the calibration curve by interpolation. Using data reduction software a 4-Parameter-Fit with lin-log coordinates for optical density and concentration is the data reduction method of choice.
Calibration This assay system is calibrated in relative arbitrary units. Calibration is related to the intemationally recognised reference sera and to IRP 97/656 (IgG) and HCAL (IgG)/ EY2C9 (IgM).

Citations

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Documents

pdf COA

pdf SDS

pdf Datasheet

QA & Reviews

Customer Reviews

References

Evidence for inhibition of low density lipoprotein oxidation and cholesterol accumulation by apolipoprotein H (beta(2)-glycoprotein I)

LIFE SCIENCES

Authors: Lin, KY; Pan, JP; Yang, DL; Huang, KT; Chang, MS; Ding, PYA; Chiang, AN

Abstract

Low density lipoprotein (LDL) oxidation and lipid accumulation are thought to enhance the progression of atherosclerosis, Apolipoprotein H (apoH) has been implicated in the development of human atherosclerosis, However, the roles of apoH in the oxidative modification of LDL and cellular accumulation of lipid constituents remained uncharacterized, In this study, the level of plasma apoH was found to be significantly associated with the oxidative susceptibility of LDL in human subjects. Plasma levels of apoH were positively correlated with the lag time but negatively correlated with LDL oxidation rate in conjugated diene formation. By using a J774 A.1 macrophage culture system, we found that apoH could not only inhibit the formation of conjugated diene and thiobarbituric acid-reactive substances, but also reduce the electrophoretic mobility of oxidized LDL. Furthermore, apoH decreased cellular accumulation of cholesterol via a reduction in cholesterol influx and an increase in cholesterol efflux, This is the first demonstration that apoH appears to have "antioxidant"-like effects on LDL oxidation. The results also suggest that apoH can inhibit the translocation of cholesterol from extracellular pools to macrophages, suggesting that apoH may play an important role in the prevention of atherosclerosis. (C) 2001 Elsevier Science Inc. All rights reserved.

Genetics of apolipoprotein H (beta(2)-glycoprotein I) polymorphism in India

ANNALS OF HUMAN BIOLOGY

Authors: Singh, P; Singh, M; Mastana, SS

Abstract

Background: Human apolipoprotein H (beta(2)-glycoprotein I, apoH, protein; APOH, gene) is a single-chain glycoprotein that has been implicated in several metabolic pathways, including lipid metabolism, coagulation and production of antiphospholipid antibodies and many disease phenotypes. The structural, molecular and genetic bases of APOH have been studied in detail but population studies, especially from the Indian subcontinent, are limited. Objective: This study seeks to enlarge our understanding of APOH genetic diversity in human populations from different regions and social groups of India. Also, we examine the level and extent of genetic variation at this locus in world populations and its utility as a population genetic marker. Subjects and methods: Blood samples from 1381 unrelated and randomly selected individuals were screened for APOH genetic polymorphism. Eleven populations from North India (Brahmins, Banias, Jat Sikhs, Khatris, Scheduled Castes, Lobanas and Rajputs), West India (Brahmin and Patels) and Central India (Brahmins and Baiga tribe) were studied for APOH polymorphism using isoelectric focusing. Allele frequencies were calculated by the gene counting method. The results were statistically evaluated using chi-square statistics for regional and ethnic variation. Genetic distances were computed on Indian populations to determine the population affinities. Correspondence analysis was used to assess ethnic variation in world populations. Results: An interesting and wide genetic variation at this locus was observed in Indian populations. The frequency distribution of three observed alleles ranged from 0.034 to 0.091 for APOH* 1, 0.852 to 0.917 for APOH* 2 and 0.027 to 0.075 for APOH*3. The world's highest APOH* 2 allele frequency was observed in the Patel (0.917) caste group from West India. Conclusions: Overall, the observed variation at this locus in Indian populations is comparable to many Caucasian populations. An analysis of world populations showed that APOH is a useful genetic marker for population and anthropological studies.