Specifications
Species Reactivity
Human; Non-human primate
Immunogen
Native human APOH
Applications
Application Notes
WB, ELISA, ELISA(Cap)
We recommend the following for sandwich ELISA (Capture - Detection):
CABT-L6552Z - CABT-L6553Z
Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
Target
Alternative Names
APOH; apolipoprotein H (beta-2-glycoprotein I); BG; B2G1; B2GP1; beta-2-glycoprotein 1; B2GPI; apo-H; beta(2)GPI; APC inhibitor; anticardiolipin cofactor; activated protein C-binding protein
Citations
Publication ()
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Activated polyreactive B cells are clonally expanded in autoantibody positive and patients with recent-onset type 1 diabetes
Nicholas CA, Tensun FA, Evans SA, Toole KP, Prendergast JE, Broncucia H, Hesselberth JR, Gottlieb PA, Wells KL, Smith MJ.
Applications: ELISA
Reactive species: Human
"Abstract: Autoreactive B cells play an important but ill-defined role in autoimmune type 1 diabetes (T1D). We isolated pancreatic islet antigen-reactive B cells from the peripheral blood of non-diabetic autoantibody-negative first-degree relatives, autoantibody-positive, and recent-onset T1D donors. Single-cell RNA sequencing analysis revealed that islet antigen-reactive B cells from autoantibody-positive and T1D donors had altered gene expression in pathways associated with B cell signaling and inflammation. Additionally, BCR sequencing uncovered a similar shift in islet antigen-reactive B cell repertoires among autoantibody-positive and T1D donors where greater clonal expansion was also observed. Notably, a substantial fraction of islet antigen-reactive B cells in autoantibody-positive and T1D donors appeared to be polyreactive, which was corroborated by analysis of recombinant monoclonal antibodies. These results expand our understanding of autoreactive B cell phenotypes during T1D and identify unique BCR repertoire changes that may serve as biomarkers for increased disease risk."
Article snippet: The cardiolipin and APOH antibodies were gifts from Elena Hsieh who obtained them from Creative Diagnostics. 
Figure 1. Antigen-reactive BCR V(D)J sequences were confirmed by ELISA
COA
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