Mouse Angiotensinogen ELISA Kit

Sample

serum, plasma, tissue homogenates and other biological fluids

Species Reactivity

Mouse

Detection Method

sELISA

Intended Use

This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of AGT in mouse serum, plasma, tissue homogenates and other biological fluids.

Contents of Kit

1. Pre-coated, ready to use 96-well strip plate, 1
2. Standard, 2
3. Detection Solution A, 1 × 12 mL
4. Detection Solution B, 1 × 12 mL
5. Wash Buffer (30× concentrate), 1 × 20 mL
6. Plate sealer for 96 wells, 2
7. Standard Diluent, 1 × 20 mL
8. TMB Substrate, 1 × 9 mL
9. Stop Solution, 1 × 6 mL
10. Instruction manual, 1

Storage

Store all components at 4°C

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, middle and highlevel AGT were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and highlevel AGT were tested on 3 different plates, 8 replicates in each plate.

CV (%) = SD/mean × 100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Detection Range

0.625-40 ng/mL. The concentrations used for creating the standard curve were 40 ng/mL, 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL.

Sensitivity

The minimum detectable dose of AGT is typically less than 0.226 ng/mL.
The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates and calculating the corresponding concentration.

Standard Curve

To make the calculation easier, we plot the O.D. value of the standard (x-axis) against the known concentration of the standard (y-axis), although concentration is the independent variable and O.D. value is the dependent variable. However, the O.D. values of the standard curve may vary according to the conditions of assay performance (e.g., operator, pipetting technique, washing technique or temperature effects), plotting the log of the data to establish a standard curve for each test is recommended. The typical standard curve below is provided for reference only.