Sample
tissues culture supernatants, blood
Detection Method
Sandwich ELISA
Intended Use
The principle of this Enzyme ImmunoAssay (EIA) is summarised on the following page: a specific monoclonal anti-Angiotensin II is immobilised on a 96 well plate. After immunological reaction with Angiotensin II and washing, the trapped molecule is covalently linked to the plate by glutaraldehyde via amino groups.
Contents of Kit
1. A covered 96 well plate, Pre-Coated with mouse anti-Angiotensin II IgG, ready to use
2. One vial of mouse anti-Angiotensin II IgG tracer, lyophilised
3. Two vials of Angiotensin II Standard, lyophilised
4. One vial of glutaraldehyde, 25% aqueous solution
5. Two vials of borane-trimethylamine
6. Two vials of Quality Control sample, lyophilised
7. Two vials of Ellman's reagent, lyophilised
8. One vial of EIA buffer, lyophilised
9. One vial of concentrated Wash buffer, liquid
10. One vial of tween 20, liquid
11. One instruction booklet
12. One template sheet
13. One well cover sheet
Storage
For more detailed information, please download the following document on our website.
Detection Range
0.5-1.5 pg/mL
Sensitivity
0.5-1.5 pg/mL
Citations
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