Angiotensin 1-7 ELISA Kit

Sample

Cell Culture Supernatants, Plasma, Serum, Tissue Homogenates

Intended Use

This kit is for the detection of Angiotensin 1-7 in Cell Culture Supernatants, Plasma, Serum, and Tissue Homogenates.

Contents of Kit

1. Coated 96-well Strip Plate: 1
2. Standard (Lyophilized): 2 vials
3. Sample Diluent: 1 vial x 20 mL
4. Assay Diluent A: 1 vial x 10 mL
5. Assay Diluent B: 1 vial x 10 mL
6. Detection Reagent A: 1 vial x 60 μL
7. Detection Reagent B: 1 vial x 120 μL
8. Wash Buffer (25x): 1 vial x 30 mL
9. TMB Substrate: 1 vial x 10 mL
10. Stop Solution: 1 vial x 10 mL
11. Adhesive Plate Sealers: 4
12. Instruction Manual: 1

Storage

Upon receipt the kit should be stored at 4°C if intended for use within 24 hours. Otherwise the Assay Plate, Standard, Detection Reagent A, and Detection Reagent B should be stored at -20°C. Avoid repeated freeze-thaw cycles. Store all other kit components at 4°C. The Substrate should never be frozen. Once individual reagents are opened it is recommended that the kit be used within 1 month. Unused Strip Plate wells should be stored at -20°C in a sealed bag containing desiccant in order to minimize exposure to moisture. Do not use the kit beyond its expiration date.

Precision

Intra-Assay CV (<5.8%)
Inter-Assay CV (<7.9%)

Detection Range

7.8–500 pg/ml

Sensitivity

Typically less than 7.8 pg/ml

Standard Curve