Anti-Human MNK1 (phospho Thr197, Thr202) monoclonal antibody

Immunofluorescent analysis of Phospho-MNK pThr197/202 in HeLa cells using a Phospho-MNK pThr197/202 recombinant rabbit monoclonal antibody at a dilution of 5 µg/mL in the absence of peptide (top left) and presence of phosphopeptide used as immunogen (top right) or non-phosphopeptide (bottom left), followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Actin was stained with Alexa Fluor 568 phalloidin. Hoechst only (blue, left), AF488 signal only (green, center) and composite image with Phalloidin (right).

Immunohistochemistry analysis of Phospho-MNK pThr197/202 in formalin-fixed, paraffin-embedded human thyroid carcinoma (top left), normal thyroid (top right) and prostate carcinoma (bottom) using a Phospho-MNK pThr197/202 monoclonal antibody at a dilution of 2 µg/mL. Staining was visualized using DAB and images were taken at a magnification of 20x. Results show nuclear and cytoplasmic staining in tumor cells and no staining in normal tissue.