Specifications
Antibody Isotype
Fab Fragment
Species Reactivity
Rabbit
Conjugate
Duocarmycin DM (Cleavable)
Applications
Application Notes
Used in cell-based cytotoxic assay.
Anti-Rabbit IgG Fc polyclonal antibody [CL-Duocarmycin] (Fab Fragment) is a secondary antibody-drug conjugates for pre-screening antibodies with a Rabbit IgG Fc moiety or recombinant Rabbit IgG Fc fusion proteins to determine their cytotoxicity as Duocarmycin DM bioconjugates. When applied in combination with tumor specific monoclonal antibodies or recombinant Fc fusion proteins, it can help determine the cytotoxic potential for these antibodies or proteins against target cell lines. The mono-valence nature of Fab secondary antibody-drug conjugates may have some advantage in certain applications than the full length IgG secondary ADC.
General Notes
SecADC™ Anti-Rabbit IgG Fc polyclonal antibody [CL-Duocarmycin] (Fab Fragment) can be used in cell-based cytotoxicity assays to pre-screening monoclonal antibodies as ADC candidates against tumor cells. This product is a Fab fragment of anti-Rabbit IgG Fc specific antibody conjugated to duocarmycin DM (DMDM) with a cleavable linker. The antibody portion is a polyclonal antibody which is specific to the Fc region of Rabbit IgGs. Duocarmycin is a cytotoxic small molecule which causes cell death by binding to the minor groove of DNA and alkylating the adenine. The cleavable linker connecting duocarmycin to the antibody is stable in extracellular fluid, but is cleaved by cathepsin in endosome once the conjugate has entered a cell via endocytosis.
Target
Alternative Names
Ig gamma 1 chain C region; Immunoglobin heavy constant gamma 1; Immunoglobulin G; IgG; IgG heavy chain; Immunoglobulin G heavy chain; IGHG1; IGHG2; IGHG3; IGHG4;
Product Background
Pathway
IL4-mediated signaling events;
Citations
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Asano, R; Shimomura, I; et al. Rearranging the domain order of a diabody-based IgG-like bispecific antibody enhances its antitumor activity and improves its degradation resistance and pharmacokinetics. MABS 6:1243-1254(2014).
Nestorova, GG; Kopparthy, VL; et al. Thermoelectric lab-on-a-chip ELISA. ANALYTICAL METHODS 7:2055-2063(2015).
COA
Certificate of Analysis
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