Dog IgG ELISA Kit

Name: Dog IgG ELISA Kit
SKU: DEIA633
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

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COA

SDS

Datasheet

Sample

Serum, plasma

Species Reactivity

Dog

Detection Method

Sandwich-ELISA

Intended Use

The dog IgG ELISA kit is designed for measurement of IgG in dog serum or plasma.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Anti Dog IgG Coated 96-well Plate	12×8 wells	2-8°C
2	HRP Conjugate Reagent	11 ml	2-8°C
3	Reference Standard (lyophilized)	1	2-8°C
4	20× Wash Solution	50 ml	2-8°C
5	10× Diluent	50 ml	2-8°C
6	TMB Reagent	11 ml	2-8°C
7	Stop Solution	11 ml	2-8°C

Storage

The test kit will remain stable for six months from the date of purchase provided that the components are stored at 4°C. The microtiter plate should be kept in a sealed bag with desiccant to minimize exposure to damp air.

Standard Curve

A typical standard curve with optical density readings at 450nm on the Y-axis against IgG concentrations on the X-axis is shown below. This curve is for the purpose of illustration only and should not be used to calculate unknowns. Each user should obtain his or her data and standard curve in each experiment.

Citations

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Datasheet

Certificate of Analysis

Safety Data Sheet

Immunoassays for Process-related Impurities

Customer Reviews

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Microbiological, Physicochemical, and Immunological Analysis of a Commercial Cashew Nut-Based Yogurt

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

Authors: Mattison, Christopher P.; Aryana, Kayanush J.; Clermont, Kristen; Prestenburg, Eric; Lloyd, Steven W.; Grimm, Casey C.; Wasserman, Richard L.

Abstract

Nut-based milks and yogurts are gaining popularity, but may not offer the same benefits as dairy yogurts to consumers. Cashew nuts often cause severe allergic reactions, and cashew nut allergens are stable to several types of processing. To compare its characteristics to dairy yogurt and characterize the effects of fermentation on the Ana o 1-3 cashew nut allergens, a commercial yogurt made from cashew nuts (Cashewgurt) was evaluated for microbiological, physiochemical, and immunological properties. Average counts for lactobacilli and Streptococcus thermophilus were greater than 10 million colony forming units per milliliter, indicating the capacity to provide a health benefit. Cashewgurt pH and viscosity values were comparable to cow milk yogurts, and it was off white in color. SDS-PAGE analysis indicated a clear reduction in Ana o 1 and 2, and immuno-assay with polyclonal anti-cashew IgG antibody and cashew-allergic IgE indicated an overall reduction in allergen content. In contrast, SDS-PAGE, mass spectrometry, immunoblot, and ELISA all revealed that Ana o 3 was relatively unaffected by the fermentation process. In conclusion, Ana o 1 and Ana o 2 are sensitive to degradation, while Ana o 3 survives lactic acid bacterial fermentation during yogurt production. The analysis presented here indicates that cashew nut yogurt is not suitable for those with cashew nut allergy.

Surface Modification of a Stable CdSeZnS/ZnS Alloy Quantum Dot for Immunoassay

JOURNAL OF NANOMATERIALS

Authors: An, Jaehyun; Huynh, Kim-Hung; Ha, Yuna; Jung, Heung Su; Kim, Hyung-Mo; Kim, Dong-Min; Kim, Jaehi; Pham, Xuan-Hung; Kim, Dong-Eun; Ho, Jin-Nyoung; Lee, Sangchul; Lee, Ho-Young; Jeong, Dae Hong; Jun, Bong-Hyun

Abstract

Quantum dots (QDs) are powerful materials in various bioapplications based on their excellent optical and electronic properties. For the application of various fields of QDs, surface modification of QDs is necessary. However, surface modification in QDs may result in a reduction in quantum yield (QY). This reduction of QY causes many weaknesses in the biological application of QDs. In this study, CdSeZnS/ZnS alloy QDs were used to prepare antibody-conjugated QDs for a sandwich immunoassay. The alloy QDs displayed a QY of 84.5% that was maintained at 83.0% (98.2% of QY was maintained) after surface modification with the anti-rabbit IgG as a model study. Surface-modified QDs successfully detected their corresponding target through antibody-antigen binding. The limit of detection was 1.1x10(2) ng mL(-1) for rabbit IgG.