Bovine IgG2 ELISA Kit

Name: Bovine IgG2 ELISA Kit
SKU: DEIA614
Rating: 5 (1 reviews)

Regulatory status: For research use only, not for use in diagnostic procedures.

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COA

SDS

Datasheet

Sample

Serum, Plasma, Colostrum, Milk

Species Reactivity

Bovine

Detection Method

Sandwich-ELISA

Intended Use

This enzyme linked immunosorbent assay (ELISA) is for the detection of Bovine IgG2 in serum, plasma, milk, and colostrum.

Contents of Kit

No.	Components	Size	Storage Conditions
1	Bovine IgG2 Pre-Coated 96-well Strip Plate	1 each	2-8°C
2	Bovine IgG2 Standard, 750 ng/vial	2 each	2-8°C
3	Bovine IgG2 Detection Antibody	12 ml	2-8°C
4	20× Dilution Buffer C	25 ml	2-8°C
5	HRP Solution C	12 ml	2-8°C
6	TMB Substrate	12 ml	2-8°C
7	Stop Solution	12 ml	2-8°C
8	20× Wash Buffer	50 ml	2-8°C
9	Sealing Tape	6 sheets

Storage

Stored at 2-8°C for 6 months from date of receipt.

General Description

There are five classes of mammalian immunoglobulins (Ig): IgA, IgD, IgE, IgG, and IgM. In cows, the Ig classes that have been identified are, IgA, IgG, IgM and IgE. No evidence of bovine IgD has been found (Naessens, 1997). Bovine IgG is further divided into two subclasses, IgG1 and IgG2. IgG is the immunoglobulin found in the highest concentration in serum. It is a relatively small Ig that exists as a monomer of two heavy and two light chain (H2L2) polypeptides linked together by disulfide bonds. Bovine IgG1 constitutes about 50% of the serum IgG in cows while IgG2 levels are just slightly less (Tizard, 1982). Serum IgG1 concentration is reported in the range of 6.0 - 15.1 mg/ml, while IgG2 levels are reported in a range from 5.0 -13.5 mg/ml (Butler, 1983). Bovine IgG1 is the predominant IgG in milk and colostrum and is found at higher concentrations than IgG2. Reported values for IgG1 in colostrum are in the range of 30.0 – 75.0 mg/ml, while IgG2 levels range from 1.9 - 4.0 mg/ml (Butler, 1983). The activities and functions of IgG1 and IgG2 appear to have similar and distinct roles in immunity. Bovine IgG2 has been shown to play an important role in neutrophil stimulation (Tao, Corbett, & Pickett, 1995).

Standard Curve

This typical standard curve was generated using the Bovine IgG2 ELISA Kit Protocol. This standard curve is for demonstration only. A standard curve must be generated for each assay.

*Suggested standard curve points are 750 ng/ml, 250 ng/ml, 83.3 ng/ml, 27.7 ng/ml, 9.26 ng/ml, 3.0 ng/ml, 1.0 ng/ml, and 0 ng/ml. Assay Range: 1.0 – 750 ng/ml

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Datasheet

Certificate of Analysis

Safety Data Sheet

Immunoassays for Process-related Impurities

Customer Reviews

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Immunoprotection against lethal effects of Crotalus durissus snake venom elicited by synthetic epitopes trapped in liposomes

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

Authors: Vaz de Melo, Patricia D.; Lima, Sabrina de Almeida; Araujo, Priscila; Santos, Raissa Medina; Gonzalez, Edgar; Belo, Andreza Alves; Machado-de-Avila, Ricardo A.; Costal-Oliveira, Fernanda; Soccol, Vanete T.; Guerra-Duarte, Clara; Rezende, Leonides; Chavez-Olortegui, Carlos

Abstract

Snakebites caused by Crotalus genus are the second most frequent in Brazil. Crotoxin is a beta-neurotoxin responsible for the main envenomation effects of Crotalus biting, while crotamine immobilizes the animal hind limbs, contributing to prey immobilization and to envenoming symptoms. As crotoxin and crotamine represent about 90% of Crotalus venom dry weight, these toxins are of great importance for antivenom therapy. In this sense, knowledge regarding the antigenicity/immunogenicity at the molecular level of these toxins can provide valuable information for the improvement of specific antivenoms. Therefore, the aims of this study are the identification of the B-cell epitopes from crotoxin and crotamine; and the characterization of the neutralizing potency of antibodies directed against the corresponding synthetic epitopes defined in the current study. Linear B-cell epitopes were identified using the Spot Synthesis technique probed with specific anti -C. d. terrificus venom horse IgG. One epitope of crotamine (F(12)PKEKICLPPSSDFGKMDCRW(32)) and three of crotoxin (L(10)LVGVEGHLLQFNKMIKFETR(30); Y(43)CGWGGRGRPKDATDRCCFVH(63) and T(118)YKYGYMFYPDSRCRGPSETC(138)) were identified. After synthesis in their soluble form, the peptides mixture correspondent to the mapped epitopes was entrapped in liposomes and used as immunogens for antibody production in rabbits. Anti-synthetic peptide antibodies were able to protect mice from the lethal activity of C. d. terrificus venom. (C) 2020 Elsevier B.V. All rights reserved.

Association among serum and salivary A. actinomycetemcomitans specific immunoglobulin antibodies and periodontitis

BMC ORAL HEALTH

Authors: Isola, Gaetano; Polizzi, Alessandro; Patini, Romeo; Ferlito, Sebastiano; Alibrandi, Angela; Palazzo, Giuseppe

Abstract

Background: The aim of this study was to assess the association between serum and salivary Immunoglobulin (Ig) Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) specific antibodies in healthy controls (HC) and periodontitis (PT) patients. Furthermore, the objectives were to determine whether PT influenced serum A. actinomycetemcomitans specific antibodies and whether serum or salivary antibodies against A. actinomycetemcomitans IgG were mediated by serum high-sensitivity c-reactive protein (hs-CRP). Methods: Fifty-three patients with periodontitis and 48 HC were enrolled in the present study. Patients were regularly examined and characterized by clinical, salivary and blood samples analyses. A. actinomycetemcomitans IgA and IgG antibodies and hs-CRP were evaluated using a commercially available kit. The Spearman Correlation Test and Jonckheere-Terpstra Test were applied in order to assess the interdependence between serum A. actinomycetemcomitans IgG antibodies and clinical periodontal parameters. To evaluate the dependence of the serum and salivary A. actinomycetemcomitans IgG levels from possible confounders, univariate and multivariable linear regression analyses were performed. Results: Compared to HC, patients with PT had significantly higher IgA [serum: PT, 1.89 (1.2-2.2) EU vs HC, 1.37 (0.9-1.8) EU (p = 0.022); saliva: PT, 1.67 (1.4-2.1) EU vs HC, 1.42 (0.9-1.6) EU (p = 0.019)] and A. actinomycetemcomitans IgG levels [serum: PT, 2.96 (2.1-3.7) EU vs HC, 2.18 (1.8-2.1) EU (p < 0.001); saliva, PT, 2.19 (1.8-2.5) EU vs HC, 1.84 (1.4-2) EU (p = 0.028)]. In PT patients, serum A. actinomycetemcomitans IgG were associated with a proportional extent of PT and tooth loss (P-trend value < 0.001). The univariate regression analysis demonstrated that PT (p = 0.013) and high hs-CRP (p < 0.001) had a significant negative effect on serum and salivary A. actinomycetemcomitans IgG levels. The multivariate regression analysis showed that PT (p = 0.033), hs-CRP (p = 0.014) and BMI (p = 0.017) were significant negative predictors of serum A. actinomycetemcomitans IgG while hs-CRP (p < 0.001) and BMI (P = 0.025) were significant negative predictors of salivary A. actinomycetemcomitans IgG. Conclusions: PT patients presented a significantly higher serum and salivary A. actinomycetemcomitans IgA and IgG compared to HC. There was a significant increase in serum A. actinomycetemcomitans IgG when patients presented a progressive extent of PT. Moreover, PT and hs-CRP were significant negative predictors of increased salivary and serum A. actinomycetemcomitans IgG levels.