Anti-IgG polyclonal antibody (DPAB0386GH)

Specifications


Host Species
Goat
Species Reactivity
Human
Immunogen
Whole human IgG
Conjugate
Unconjugated

Target


Alternative Names
Ig gamma 1 chain C region; Immunoglobin heavy constant gamma 1; Immunoglobulin G; IgG; IgG heavy chain; Immunoglobulin G heavy chain
Entrez Gene ID

Product Background


Pathway
IL4-mediated signaling events

Citations


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References


Increased Risk of Toxoplasma gondii Infection in Patients with Colorectal Cancer in Eastern China: Seroprevalence, Risk Factors, and a Case-Control Study

BIOMED RESEARCH INTERNATIONAL

Authors: Yu, Yang; Guo, Dong; Qu, Tingting; Zhao, Shuchao; Xu, Chang; Wang, Longlong; Wang, Zhongjun; Fu, Haiyang; Zhang, Xiangyan; Zhou, Na

The aim of this study was to explore the epidemiology ofToxoplasma gondiiinfection in patients with colorectal cancer (CRC) in eastern China. Therefore, 287 primary CRC patients and 287 age-matched healthy control subjects were recruited to estimate the seroprevalence ofT. gondiiand identify the risk factors of infection. Enzyme-linked immunoassays were used to test for anti-T. gondiiimmunoglobulin G (IgG) and IgM antibodies. Forty-six (16%) samples were positive for anti-T. gondiiIgG antibodies in patients with CRC, compared with 26 (9.1%) in the healthy controls, a significant difference (P=0.007). By contrast, eight (2.8%) patients tested positive forT. gondiiIgM antibodies, compared with three (1.1%) in the controls, a difference that was not significant (P=0.13). Multivariable backward stepwise logistic regression analysis revealed that a rural residence (OR 2.83; 95% CI 1.15-7.01;P=0.024) and treatment with chemotherapy (OR 2.16; 95% CI 1.02-4.57;P=0.045) were risk factors forT. gondiiinfection in patients with CRC. Thus,T. gondiiinfection is serious in patients with CRC, and a rural residence and treatment with chemotherapy are independent risk factors for infection by this parasite. Therefore, medical professionals should be aware of this pathogen in patients with CRC, and the causes ofT. gondiiinfection in these patients need to be explored further.

A lab in a bento box: an autonomous centrifugal microfluidic system for an enzyme-linked immunosorbent assay

ANALYTICAL METHODS

Authors: Abe, Takaaki; Okamoto, Shunya; Taniguchi, Akinobu; Fukui, Michiyasu; Yamaguchi, Akinobu; Utsumi, Yuichi; Ukita, Yoshiaki

In this paper, we report on the demonstration of a portable immunoassay system consisting of a small centrifugal microfluidic device driver (bento box) and a centrifugal microfluidic device made of polypropylene and fabricated by injection molding. The bento box consists of a cheap DC motor and an Arduino microcontroller. It has a simple structure and is the size of a bento box, that is, 150 x 150 x 100 (W x D x H) mm(3). The developed device can automatically execute an enzyme-linked immunosorbent assay (ELISA) process under a steady rotating condition because it was designed based on the principle of CLOCK, which we previously presented. Here, we first executed an ELISA using a system consisting of the bento box and a device made of polydimethylsiloxane (PDMS) and compared it with a servo-controlled device driver. It was confirmed that the results of the bento box were consistent with those of the servo-controlled device driver. The limit of detection (LOD) using the bento box was 0.759 ng ml(-1). Therefore, the controllability of the bento box was demonstrated. Next, we evaluated the injection-molded device through multi-step fluid control. We confirmed, through real-time observation of the device, that accurate flow control in the designed ELISA procedure was executed. Lastly, ELISA was employed for the measurements of mouse IgG using the system consisting of the bento box and the polypropylene device. The system performed all fluidic controls within 12 min; we confirmed the specificity of the system, and the LOD was 0.320 ng ml(-1).

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