Specifications
Conjugate
Duocarmycin DM (Cleavable)
Applications
Application Notes
Used in cell-based cytotoxic assay.
Anti-Mouse IgG Fc polyclonal antibody [CL-Duocarmycin] is a secondary antibody-drug conjugates for pre-screening antibodies with a Mouse IgG Fc moiety or recombinant Mouse IgG Fc fusion proteins to determine their cytotoxicity as Duocarmycin bioconjugates. It alone displays no obvious toxicity against multiple cell lines at 1 ug/ml (6.6 nM) or lower concentration. When applied in combination with tumor specific monoclonal antibodies or recombinant Fc fusion proteins, it can help determine the cytotoxic potential for these antibodies or proteins against target cell lines.
General Notes
SecADC™ Anti-Mouse IgG Fc polyclonal antibody [CL-Duocarmycin] can be used in cell-based cytotoxicity assays to pre-screening monoclonal antibodies as ADC candidates against tumor cells. This product is anti-Mouse IgG Fc specific antibody conjugated to duocarmycin DM (DMDM) with a cleavable linker. The antibody portion is a polyclonal antibody which is specific to the Fc region of Mouse IgGs. Duocarmycin is a cytotoxic small molecule which causes cell death by binding to the minor groove of DNA and alkylating the adenine. The cleavable linker connecting duocarmycin to the antibody is stable in extracellular fluid, but is cleaved by cathepsin in endosome once the conjugate has entered a cell via endocytosis.
Target
Alternative Names
Ig gamma 1 chain C region; Immunoglobin heavy constant gamma 1; Immunoglobulin G; IgG; IgG heavy chain; Immunoglobulin G heavy chain; IGHG1; IGHG2; IGHG3; IGHG4;
Product Background
Pathway
IL4-mediated signaling events;
Citations
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Akter, R; Rhee, CK; et al. A highly sensitive quartz crystal microbalance immunosensor based on magnetic bead-supported bienzymes catalyzed mass enhancement strategy. BIOSENSORS & BIOELECTRONICS 66:539-546(2015).
Reinhardt, A; Yang, Y; et al. Antigenic Potential of a Highly Conserved Neisseria meningitidis Lipopolysaccharide Inner Core Structure Defined by Chemical Synthesis. CHEMISTRY & BIOLOGY 22:38-49(2015).
COA
Certificate of Analysis
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