Human Ceruloplasmin ELISA Kit

Sample

Plasma, serum, urine, milk, saliva and cell culture samples

Intended Use

This human ceruloplasmin antigen assay is intended for the quantitative determination of total ceruloplasmin antigen in human plasma, serum, urine, milk, saliva and cell culture samples. For research use only.

Storage

Store all kit components at 4°C upon arrival. Return any unused microplate strips to the plate pouch with desiccant. Reconstituted standards and primary may be stored at -80°C for later use. Do not freeze-thaw the standard and primary antibody more than once. Store all other unused kit components at 4°C. This kit should not be used beyond the expiration date.

Sensitivity

The minimum detectable dose (MDD) was determined by adding two standard deviations to the mean optical density value of twenty zero standard replicates (range OD450: 0.052-0.061) and calculating the corresponding concentration. The MDD was 0.333 ng/mL.

General Description

Ceruloplasmin (aka Ferroxidase I) is a 132kDa 1,046 amino acid glycoprotein which carries 95% of serum copper by binding 6 cupric ions per molecule [1]. Levels are decreased in Wilson's Disease (hepatolenticular degeneration) and heritable aceruloplasminemia leading to iron accumulation in the liver or brain from impaired iron homeostasis [2].