Rabbit Anti-Human CSK monoclonal antibody, clone D.789.5

Spaceborne multi-temporal interferometric synthetic aperture radar (MT-InSAR) is a monitoring technique capable of extracting line of sight (LOS) cumulative surface displacement measurements with millimeter accuracy. Several improvements in the techniques and datasets quality led to more effective, near real time assessment and response, and a greater ability of constraining dynamically changing physical processes. Using examples of the COSMO-SkyMed (CSK) system, we present a methodology that bridges the gaps between MT-InSAR and the relative stiffness method for tunnel-induced subsidence damage assessment. The results allow quantification of the effect of the building on the settlement profile. As expected the greenfield deformation assessment tends to provide a conservative estimate in the majority of cases (similar to 71% of the analyzed buildings), overestimating tensile strains up to 50%. With this work we show how these two techniques in the field of remote sensing and structural engineering can be synergistically used to complement and replace the traditional ground based analysis by providing an extended coverage and a temporally dense set of data.

Full T cell activation depends on stimulation of the TCR in conjunction with a costimulatory receptor. The involvement of costimulatory molecules is potent, and a mechanistic understanding of how downstream signaling is regulated is required to fully understand T cell responsiveness. In this study, a proteomic approach was taken to identify the interactomes of the coreceptors CD2 and CD28. These coreceptors are both positive regulators of T cell activation, but CD28 less potently induces TCR-proximal signaling. C-terminal Src kinase (CSK), a negative regulator of TCR signaling, was identified as a specific and direct interactor only of activated CD28. CSK is recruited to CD28 upon T cell activation, and the in vitro kinase activity of CSK is enhanced in the presence of phosphorylated CD28. Interruption of the CSK/CD28 interaction prior to TCR/CD28 costimulation induces a signaling response which mimics the more potent CD2-induced TCR-proximal pathway activation. Thus, CD28 functions as a novel adaptor protein for CSK, and CSK regulates signaling downstream of CD28.