Intended Use
The CSK ELISA Kit is a convenient, lysate-free, high throughput and sensitive assay kit that can detect CSK protein expression profile in cells. The kit can be used for measuring the relative amounts of CSK in cultured cells as well as screening for the effects that variuos treatments, inhibitors (ie siRNA or chemicals), or activators have on CSK.
Contents of Kit
1. 96-Well Cell Culture Clear-Bottom Microplate, 1 Plate
2. 10× TBS, 30 ml (10×)
3. Quenching Buffer, 24 ml (1×)
4. Blocking Buffer, 50 ml (1×)
5. 15× Wash Buffer, 60 ml (15×)
6. 100× Anti- CSK Antibody, 60 μl (100×)
7. 100× Anti-GAPDH Antibody, 60 μl (100×)
8. HRP-Conjugated Anti-Rabbit IgG Antibody, 6 ml (1×)
9. HRP-Conjugated Anti-Mouse IgG Antibody, 6 ml (1×)
10. Primary Antibody Diluent, 12 ml (1×)
11. Ready to Use Substrate, 12 ml (1×)
12. Stop Solution, 12 ml (1×)
13. Crystal Violet Solution, 6 ml (1×)
14. SDS Solution, 24 ml (1×)
15. Adhesive Plate Seals, 2 Seals