Rabbit Anti-Human c-Cbl (phospho Tyr774) monoclonal antibody, clone 9I5M2

Methylmalonic acidemia (MMA) is a severe, heterogeneous disorder of methylmalonate and cobalamin (cbl; vitamin B12) metabolism with a poor prognosis that can cause brain damage. Identifying the magnetic resonance imaging (MRI) findings of MMA might help to make accurate diagnoses earlier in the disease course and exploring the relationship between neuropsychological scores and MRI findings, when therapy is more effective and to improve therapeutic efficacy. Cerebral MRI studies from 37 children with MMA were evaluated by a neuroradiologist. Clinical and imaging data were collected from each patient. All tests were performed during routine investigations and in accordance with the ethical principles of the Declaration of Helsinki. Informed consent was obtained from the guardians of all patients for inclusion in the study. The most common and significant findings were periventricular white matter changes (78.4%), ventricular dilation (29.7%) and cerebral atrophy (40.5%). According to the developmental quotient, the 37 patients were divided into the normal intelligence subgroup (NI, developmental quotient >= 85) and the low intelligence subgroup (LI, developmental quotient<85). The incidence of corpus callosal thinning, cortical atrophy, subcortical white matter changes, and ventricular dilation (grades 0-3) was significantly higher in the LI subgroup than in the NI subgroup (P<0.05). The incidence of no-mild and moderate-severe ventricular dilation was significantly higher in the LI subgroup than in the NI subgroup (P<0.05). Ventricular dilatation, cerebral atrophy, white matter changes, and corpus callosal thinning are the main MRI abnormalities in MMA patients, and these manifestations are significantly correlated with delayed development in children.

This work describes the design, building, and testing of miniature, low-cost LED-LDR colorimeters for absorption-spectrophotometry experiments. It also describes using these colorimeters for simplified context-based-learning (CBL) activities in school teaching laboratories and for public-engagement events. The colorimeters are simple and robust, performing well against a conventional benchtop UV-vis spectrophotometer. Data collection and analysis is quick and intuitive, allowing for efficient turnaround between groups and making it particularly useful at public-engagement events. The experiments and activities described here are based on real-life examples to emphasize the usefulness of chemical analysis and quantification in everyday life.