Sample
serum, plasma, cell culture supernatant and other biological samples.
Species Reactivity
Bovine
Intended Use
In vitro quantitative determination of HSP70 concentrations in serum, plasma, cell culture supernatant and other biological samples.
Storage
2-8°C (for sealed box), please do not freeze! See kit label for expiry date
Performance Characteristics
StabilityPerform the stability test for the sealed kit at 37°C and 2-8°C and get relevant data.
Precision
Intra-assay Precision: samples with low, medium and high concentration are tested 20 times on same plate.
Inter-assay Precision: samples with low, medium and high concentration are tested 20 times on three different plates.
Detection Range
0.313-20ng/ml
Standard Curve
This product has been tested by Quality Control Department and meets performance specifications mentioned in the manual. (The humidity in the laboratory is 20%-60%, and the temperature is 18°C -25°C. TMB was balanced to 37°C before color development, and incubated at 37°C for 15 minutes in the dark after adding the enzyme label plate holes.)
The following assay data are provided for reference, since experimental environment and operation are different. The establishment of standard curve depends on your own assay.

Citations
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Liu, N; Deguchi, K; et al. In Vivo Optical Imaging of Early-Stage Apoptosis in Mouse Brain After Transient Cerebral Ischemia. JOURNAL OF NEUROSCIENCE RESEARCH 88:3488-3497(2010).
Zeng, RH; Zhang, ZY; et al. Protective effect of a RSV subunit vaccine candidate G1F/M2 was enhanced by a HSP70-Like protein in mice. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 377:495-499(2008).